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Human CD23/Fc epsilon  RII Antibody

Catalog #: MAB123
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MAB123
MAB123-SP
Detection of CD23/Fc epsilon RII in Human Blood Lymphocytes by Flow Cytometry.
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Human CD23/Fc epsilon  RII Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD23/Fc epsilon RII in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 138628
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD23/Fc epsilon RII
Met150-Ser321
Accession # P06734
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD23/Fc epsilon  RII (Catalog # 123-FE)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Lymph Nodes
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of human IgE to immobilized Recombinant Human CD23/Fc epsilon  RII (Catalog # 123-FE) coated at 2 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD23/Fce RII antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD23/Fc epsilon RII in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Mouse Anti-Human CD23/Fce RII Monoclonal Antibody (Catalog # MAB123) or (B) Mouse IgG1Isotype Control (MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry View Larger

Detection of CD23/Fc epsilon  RII in Human Lymph Nodes. CD23/Fc epsilon  RII was detected in immersion fixed paraffin-embedded sections of Human Lymph Nodes using Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB123) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD23/Fc epsilon RII

CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily (1‑5). Human CD23 is a 47 kDa type II transmembrane glycoprotein that is expressed by a wide variety of cell types (6‑10). The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography (3, 11). The lectin domain binds both protein and carbohydrate in an apparently Ca++ independent manner (11). The coiled-coil region contributes to oligomerization (11, 12). The lectin domain in human CD23 (aa 162‑284) is 64%, 62% and 68% aa identical to the lectin domains in mouse, rat and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites (6, 12). The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis (13). The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4 (6). Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium (6, 14, 15). At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera (16). Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity (16‑18). Cleavage usually occurs between aa 150‑160 (7, 8). It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c (19‑21). CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release (21). On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (11).

References
  1. Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
  2. Heyman, B. (2000) Annu. Rev. Immunol. 18:709.
  3. Bajorath, J. and A. Aruffo (1996) Protein Sci. 5:240.
  4. Drickamer, K. (1993) Curr. Opin. Struct. Biol. 3:393.
  5. Drickamer, K. (1999) Curr. Opin. Struct. Biol. 9:585.
  6. Yokota, A. et al. (1988) Cell 55:611.
  7. Ludin, C. et al. (1987) EMBO J. 6:109.
  8. Ikuta, K. et al. (1987) Proc. Natl. Acad. Sci. USA 84:819.
  9. Kikutani, H. et al. (1986) Cell 47:657.
  10. Letellier, M. et al. (1988) J. Immunol. 141:2374.
  11. Hibbert, R.G. et al. (2005) J. Exp. Med. 202:751.
  12. Beavuil, A.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:753.
  13. Yokota, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5030.
  14. Belleau, J.T. et al. (2005) Clin. Mol. Allergy 3:6.
  15. Tu, Y. et al. (2005) Gastroenterology 129:928.
  16. Marolewski, A.E. et al. (1998) Biochem. J. 333:573.
  17. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  18. Karagiannis, S.N. et al. (2001) Immunology 103:319.
  19. Aubry, J-P. et al. (1992) Nature 358:505.
  20. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
  21. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.
Long Name
Fc epsilon Receptor II
Entrez Gene IDs
2208 (Human); 14128 (Mouse); 171075 (Rat)
Alternate Names
BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor

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Citation for Human CD23/Fc epsilon  RII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts Fc?RI interaction
    Authors: F Jabs, M Plum, NS Laursen, RK Jensen, B Mølgaard, M Miehe, M Mandolesi, MM Rauber, W Pfützner, T Jakob, C Möbs, GR Andersen, E Spillner
    Nat Commun, 2018-01-02;9(1):7.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development (Capture)

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