Human CD3+ T Cell Enrichment Column, Large

Human CD3+ T Cell Enrichment Column, Large Summary

T Cell Enrichment Columns

View Full Assay Principle

Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3⁺ is a universal marker for T cells. Total CD3⁺ cell recovery ranges between 30% and 47% of initial CD3⁺ cells loaded. The purity of recovered cells ranges from 86% to 92%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

• Available for human, mouse, and rat cells
• Purifies CD3⁺ T cells to 86 – 92%
• Based upon gravitational flow
• Simple procedure
• No specialized equipment required

Kit Contents

• Cell Selection Column
• 10X Column Buffer

Specifications

Product Datasheets

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Related Research Areas

• Acute Lymphoblastic Leukemia
• Conventional/Classical Dendritic Cells
• gamma delta T Cells
• Inflammatory/Monocyte-derived Dendritic Cells
• Isolation, Culture, and Identification of Natural Killer Cells
• ITIM/ITAM Immunoreceptors and Related Molecules
• Langerhans and Dermal Dendritic Cells
• Leukocyte Receptors/Markers in Allergy and Asthma
• Leukocyte Receptors/Markers in Tfh Cell Differentiation and Function
• Lymphoid Lineage Markers
• Natural Killer T (NKT) Cells
• Plasmacytoid Dendritic Cells
• Regulatory T Cells (Tregs)
• T Cell Antigen Recognition
• Th1 Cells
• Th17 Cells
• Th2 Cells
• Th22 Cells
• Th9 Cells