Human CD31/PECAM-1 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Membrane-Associated Staining protocol.
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CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
Product Datasheets
Citations for Human CD31/PECAM-1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Cancer-associated fibroblast-derived WNT2 increases tumor angiogenesis in colon cancer
Authors: Daniela Unterleuthner, Patrick Neuhold, Katharina Schwarz, Lukas Janker, Benjamin Neuditschko, Harini Nivarthi et al.
Angiogenesis
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GATA6 regulates anti-angiogenic properties in human cardiac fibroblasts via modulating LYPD1 expression
Authors: Masuda, S;Matsuura, K;Shimizu, T;
Regenerative therapy
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
Cells, 2021-12-10;10(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of a large-scale vascular bed for the in�vitro creation of three-dimensional cardiac tissue
Authors: A Inui, H Sekine, K Sano, I Dobashi, A Yoshida, K Matsuura, E Kobayashi, M Ono, T Shimizu
Regen Ther, 2019-10-22;11(0):316-323.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture
Authors: S Masuda, K Matsuura, T Shimizu
Regen Ther, 2018-07-07;9(0):1-9.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection, ICC -
Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
Sci Rep, 2018-04-20;8(1):6331.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
Authors: Norton, Kerri-An, Han, Zheyi, Popel, Aleksand, Pandey, Niranjan
Onco Targets Ther, 2014-09-09;7(0):1571-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
FASEB J, 2013-11-13;28(2):740-51.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
2013-09-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Flow Cytometry -
Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012-10-09;7(10):e47187.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Signaling hierarchy regulating human endothelial cell development.
Authors: Kelly MA, Hirschi KK
Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro
Authors: Xiaoyu Xing, Zhiyuan Zhang, Liang Zhong, Guanqun Ju, Xiangyu Zou, Yingjian Zhu et al.
Experimental and Therapeutic Medicine
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