Human CD34 PE-conjugated Antibody

Name: Human CD34 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB72271P

Catalog #: FAB72271P Datasheet / COA / SDS

Discontinued Product

FAB72271P has been discontinued.

View all CD34 products.

Detection of CD34 in KG‑1 Human Cell Line by Flow Cytometry.

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Human CD34 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse, rat, or porcine CD34 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 756510

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD34
Ser32-Thr290
Accession # P28906

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD34 in KG‑1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB72271P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1