Human CD38 Antibody Summary
Met1-Ile300
Accession # P28907
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD38 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, RPMI 8226 human multiple myeloma cell line, and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB24041) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific and diffuse band was detected for CD38 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CD38 in human tonsil. CD38 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB24041) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD38
CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).
- Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
- Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
- Sun, L. et al. (1999) J. Cell Biol. 146:1161.
- Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
- Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Product Datasheets
Citations for Human CD38 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Mitochondrial oxidative capacity and NAD+ biosynthesis are reduced in human sarcopenia across ethnicities
Authors: Eugenia Migliavacca, Stacey K. H. Tay, Harnish P. Patel, Tanja Sonntag, Gabriele Civiletto, Craig McFarlane et al.
Nature Communications
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Flavonoid apigenin is an inhibitor of the NAD+ ase CD38: implications for cellular NAD+ metabolism, protein acetylation, and treatment of metabolic syndrome.
Authors: Escande, Carlos, Nin, Veronica, Price, Nathan L, Capellini, Verena, Gomes, Ana P, Barbosa, Maria Th, O'Neil, Luke, White, Thomas A, Sinclair, David A, Chini, Eduardo
Diabetes, 2012-11-19;62(4):1084-93.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptional response of human dendritic cells to Borrelia garinii--defective CD38 and CCR7 expression detected.
Authors: Hartiala P, Hytonen J, Pelkonen J, Kimppa K, West A, Penttinen MA, Suhonen J, Lahesmaa R, Viljanen MK
J. Leukoc. Biol., 2007-04-17;82(1):33-43.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
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