Human CD44s Pan Specific Antibody Summary
Gln21-Pro220
Accession # P16070
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD44 in Human Lymph Node via seqIF™ staining on COMET™ CD44 Antibody was detected in immersion fixed paraffin-embedded sections of human Lymph Node using Mouse Anti-Human CD44, Monoclonal Antibody (Catalog # MAB7045) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Detection of Human CD44 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CD44 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
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CD44 in Human PBMCs. CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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CD44 in Human Tonsil. CD44 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of CD44 in PC‑3 (Positive) & Daudi (Negative). CD44 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD44
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1‑10, exons 6‑15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s or CD44H, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
- Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
- Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
- Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
- Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
- Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
- Nakamura, H. et al. (2004) Cancer Res. 64:876.
- Murakami, D. et al. (2003) Oncogene 22:1511.
- Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Product Datasheets
Citations for Human CD44s Pan Specific Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Cellular adaptation to cancer therapy along a resistance continuum
Authors: França, GS;Baron, M;King, BR;Bossowski, JP;Bjornberg, A;Pour, M;Rao, A;Patel, AS;Misirlioglu, S;Barkley, D;Tang, KH;Dolgalev, I;Liberman, DA;Avital, G;Kuperwaser, F;Chiodin, M;Levine, DA;Papagiannakopoulos, T;Marusyk, A;Lionnet, T;Yanai, I;
Nature
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Transplantable human motor networks as a neuron-directed strategy for spinal cord injury
Authors: Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al.
iScience
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Mammospheres of letrozole-resistant breast cancer cells enhance breast cancer aggressiveness
Authors: JR Patel, KM Gallegos, RR Walker, AM Davidson, I Davenport, SL Tilghman
Oncology Letters, 2021-06-28;22(2):620.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of resistant triple-negative breast cancer cells with low-dose 6-mercaptopurine and 5-azacitidine
Authors: B Singh, VN Sarli, A Lucci
Oncotarget, 2021-03-30;12(7):626-637.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluation of 6-mercaptopurine in a cell culture model of adaptable triple-negative breast cancer with metastatic potential
Authors: Balraj Singh, Vanessa N. Sarli, Hannah E. Kinne, Anna Shamsnia, Anthony Lucci
Oncotarget
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Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
PLoS ONE, 2019-05-02;14(5):e0215990.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
A Preliminary Study: Human Fibroid Stro-1+/CD44+ Stem Cells Isolated From Uterine Fibroids Demonstrate Decreased DNA Repair and Genomic Integrity Compared to Adjacent Myometrial Stro-1+/CD44+ Cells
Authors: Lauren E. Prusinski Fernung, Ayman Al-Hendy, Qiwei Yang
Reproductive Sciences
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SALL4 promotes gastric cancer progression through activating CD44 expression
Oncogenesis, 2016-11-07;5(11):e268.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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MAB7045 was used as the capture antibody along with BBA10 as the detection antibody. Recombinant human CD44 Fc Chimera (3660-CD) was used as the calibrator material. Human serum and plasma samples were diluted 1:5 and all were quantifiable. Parallelism looked good.
