Human CD45RA Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD45RA

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA.  CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene.  In humans, CD45RA is one of six isoforms of the CD45 complex.  These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC.  CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells.  CD45RA contains exon 4 but lacks both exons 5 and 6.  CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons.  Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells.  Other cell types such as activated monocytes and B cells can also express CD45RO.  Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Airway Inflammation and Remodeling
• Basophils
• Eosinophils
• Epigenetics
• Granulocyte Markers
• Mesenchymal Stem Cell Markers
• Monocyte Markers
• Negative Regulators of the Jak/STAT Pathway
• Neutrophils
• Osteoblast and Osteoclast Markers
• Phosphatases and Regulators
• Phosphatases in the Akt Pathway
• Regulatory T Cells (Tregs)
• Search Kinases and Phosphatases
• T Cell Antigen Recognition