Human CD45RA Fluorescein-conjugated Antibody

Catalog #: FAB11444F Datasheet / COA / SDS

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FAB11444F-100

Detection of CD45RA in PBMC lymphocytes by Flow Cytometry

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Human CD45RA Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects CD45RA in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1069433

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Whole cell transfectant expressing CD45RA
Accession # P08575

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD45RA in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD45RO Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB10642R) and either (A) Mouse Anti-Human CD45RA Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11444F) or (B) isotype control antibody (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45RA

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA. CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene. In humans, CD45RA is one of six isoforms of the CD45 complex. These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC. CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells. CD45RA contains exon 4 but lacks both exons 5 and 6. CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons. Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells. Other cell types such as activated monocytes and B cells can also express CD45RO. Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Long Name

CD45R, A isoform

Entrez Gene IDs

5788 (Human)

Alternate Names

B220; CD45 antigen; CD45R; CD45RA; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide