Human CD45RB Antibody

Catalog #: MAB11434 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11434-100
MAB11434-SP

Detection of CD45RB in PBMC lymphocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD45RB Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD45RB in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1068228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD45RB
Gln26-Lys463
Accession # P08575

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

PBMC lymphocytes

Immunocytochemistry

8-25 µg/mL

Human PBMCs (positive) and absent in HEK293 human embryonic kidney cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD45RB in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stainedwith CD45RA and either (A)Mouse Anti-Human CD45RBMonoclonal Antibody (Catalog #MAB11434) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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Detection of CD45RB in Human PBMCs (positive) and HEK293 (negative) cells. CD45RB was detected in immersion fixed Human PBMCs (positive) and absent in HEK293 human embryonic kidney cells (negative) using Mouse Anti-Human CD45RB Monoclonal Antibody (Catalog # MAB11434) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45RB

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes and the shortest form, CD45R0 is expressed on memory cells (5). Isoform CD45RB contains exon 5 (B exon) in the extracellular domain, which shares 41% and 40% homology with the ECD of mouse and rat CD45RB. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Interaction of galaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates of CD45 (4, 9). Within the immunological synapse, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References

Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
Ralph, S.J. et al. (1987) EMBO J. 6:1251.
Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
McNiell, L. et al. (2007) Immunity 27:425.
Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
Zhu, J.W. et al. (2008) Immunity 28:183.
Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
Cross, J.L. et al. (2008) J. Immunol. 180:8020.

Long Name

CD45R, B isoform

Entrez Gene IDs

5788 (Human); 19264 (Mouse)

Alternate Names

B220; CD45 antigen; CD45R; CD45RB; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide