Human CD45RB APC-conjugated Antibody Summary
Gln26-Lys463
Accession # P08575
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD45RB in PBMC lymphocytes cells by Flow Cytometry. PBMC lymphocytes were stained with either (A) Mouse Anti-Human CD45RB APC‑conjugated Monoclonal Antibody (Catalog # FAB11434A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD45RB
CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes and the shortest form, CD45R0 is expressed on memory cells (5). Isoform CD45RB contains exon 5 (B exon) in the extracellular domain, which shares 41% and 40% homology with the ECD of mouse and rat CD45RB. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Interaction of galaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates of CD45 (4, 9). Within the immunological synapse, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).
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- Cross, J.L. et al. (2008) J. Immunol. 180:8020.
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