Human CD7 Antibody

Catalog #: MAB7579 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7579
MAB7579-SP

Detection of CD7 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD7 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 848438

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived human CD7
Ala26-Pro180
Accession # P09564

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human CD7 Monoclonal Antibody (Catalog # MAB7579) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD7

CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34⁺ myeloid and lymphoid progenitor cells, memory CLA- CD45RA⁺ T cells, and CD56⁺ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

Entrez Gene IDs

924 (Human); 12516 (Mouse); 303747 (Rat); 102124399 (Cynomolgus Monkey)

Alternate Names

CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein