Human CD7 APC-conjugated Antibody

Name: Human CD7 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB7579A

Catalog #: FAB7579A Datasheet / COA / SDS

Discontinued Product

FAB7579A has been discontinued.

View all CD7 products.

Detection of CD7 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD7 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD7 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 848438

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived human CD7
Ala26-Pro180
Accession # P09564

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD7 APC-conjugated Monoclonal Antibody (Catalog # FAB7579A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD7

CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34⁺ myeloid and lymphoid progenitor cells, memory CLA- CD45RA⁺ T cells, and CD56⁺ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

Entrez Gene IDs

924 (Human); 12516 (Mouse); 303747 (Rat); 102124399 (Cynomolgus Monkey)

Alternate Names

CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein