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Human CD96v2 Antibody

Catalog #: MAB6199
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6199
MAB6199-SP
Detection of CD96 in Human PBMCs by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD96v2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD96 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 628211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD96
Val22-Asp501
Accession # NP_005807
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD96 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD96 in Human PBMCs by Flow Cytometry. Human peripheral blood cells were stained with Human CD96 Monoclonal Antibody (Catalog # MAB6199) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4+ and CD8+ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155+ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Entrez Gene IDs
10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)
Alternate Names
CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile

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Citation for Human CD96v2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies
    Authors: A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha
    JCI Insight, 2022-10-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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