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Human CDX2 Antibody

Catalog #: AF3665
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3665
AF3665-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CDX2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDX2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CDX2
Asn54-Thr185
Accession # Q99626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CDX2
Immunocytochemistry
5-15 µg/mL
Immersion fixed BG01V human embryonic stem cells differentiated to trophectoderm using Mouse Embryonic Fibroblast (MEF) Conditioned Media (Catalog # AR005) with 10 ng/mL recombinant human FGF-basic (Catalog # 233-FB-025) and 100 ng/mL recombinant human BMP-4 (Catalog # 314-BP-010) for 7 days

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDX2

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

Long Name
Caudal-type Homeobox Transcription Factor 2
Entrez Gene IDs
1045 (Human); 12591 (Mouse); 66019 (Rat)
Alternate Names
caudal type homeo box transcription factor 2; caudal type homeobox 2; Caudal-type homeobox protein 2; CDX2; CDX3; CDX-3; CDX3caudal type homeobox transcription factor 2; homeobox protein CDX-2

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Citations for Human CDX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Highly conserved and cis-acting lncRNAs produced from paralogous regions in the center of HOXA and HOXB clusters in the endoderm lineage
    Authors: Neta Degani, Yoav Lubelsky, Rotem Ben-Tov Perry, Elena Ainbinder, Igor Ulitsky
    PLOS Genetics
  2. A single-cell transcriptome atlas of human euploid and aneuploid blastocysts
    Authors: Wang, S;Leng, L;Wang, Q;Gu, Y;Li, J;An, Y;Deng, Q;Xie, P;Cheng, C;Chen, X;Zhou, Q;Lu, J;Chen, F;Liu, L;Yang, H;Wang, J;Xu, X;Hou, Y;Gong, F;Hu, L;Lu, G;Shang, Z;Lin, G;
    Nature genetics
    Species: Human
    Sample Types: Embryo
    Applications: Immunohistochemistry
  3. Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids
    Authors: AC Silva, OB Matthys, DA Joy, MA Kauss, V Natarajan, MH Lai, D Turaga, AP Blair, M Alexanian, BG Bruneau, TC McDevitt
    Cell Stem Cell, 2021-12-02;28(12):2137-2152.e6.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  4. Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
    Authors: E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek
    Int J Mol Sci, 2020-07-14;21(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Gastrointestinal transcription factors drive lineage-specific developmental programs in organ specification and cancer
    Authors: R Francis, H Guo, C Streutker, M Ahmed, T Yung, PB Dirks, HH He, TH Kim
    Sci Adv, 2019-12-11;5(12):eaax8898.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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