Human CEACAM-20 Antibody Summary
Gln31-Gly450
Accession # Q6UY09-1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CEACAM-20 in HEK293 Human Cell Line Transfected with Human CEACAM-20 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-20 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human CEACAM-20 Monoclonal Antibody (Catalog # MAB9416) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-20
Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 amino acid (aa) extracellular domain (ECD), a 21 aa helical transmembrane segment, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). Within the ECD, human CEACAM-20 shares 64% and 62% aa identity with the mouse and rat CEACAM-20, respectively. The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought to act as a physiological substrate for SAP-1 in the intestinal epithelium (10).
- Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
- Zhang, H. et al. (2013) PLoS ONE 8:e53359.
- Zebhauser, R. et al. (2005) Genomics 86:566.
- Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
- Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
- Murata, Y. et al. (2015) PNAS E4264.
- Obrink, B. (1997) Curr Opin Cell Biol 9:616.
- Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283.
- Kitamura, Y. et al. (2015) Genes to Cells 20:578.
- Kotani, T. et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.
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