Human CEACAM-5/CD66e Antibody

Catalog #: MAB41282 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB41282-100
MAB41282-SP

Human CEACAM‑5/CD66e ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEACAM-5/CD66e Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-5/CD66e in sandwich ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 487610

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human CEACAM-5/CD66e
Lys35-Ala685
Accession # Q8N4D0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human CEACAM‑5/CD66e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF41281).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CEACAM-5/CD66e DuoSet ELISA Kit (Catalog # DY4128) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human CEACAM‑5/CD66e ELISA Standard Curve. Recombinant Human CEACAM‑5/CD66e protein was serially diluted 2-fold and captured by Mouse Anti-Human CEACAM‑5/CD66e Monoclonal Antibody (Catalog # MAB41282) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human CEACAM‑5/CD66e Antigen Affinity-purified Polyclonal Antibody ()AF41281 was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor
(2-4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a
calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6-8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14-16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

Zebhauser, R. et al. (2005) Genomics 86:566.
Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
Garcia, M. et al. (1991) Cancer Res. 51:5679.
Stern, N. et al. (2005) J. Immunol. 174:6692.
Benchimol, S. et al. (1989) Cell 57:327.
Zhou, H. et al. (1993) J. Cell Biol. 122:951.
Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
Ordonez, C. et al. (2000) Cancer Res. 60:3419.
Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
Chen, T. et al. (1997) J. Exp. Med. 185:1557.
Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100