Human CEACAM-5/CD66e Antibody

Name: Human CEACAM-5/CD66e Antibody
Brand: R&D Systems
SKU: MAB4128

Catalog #: MAB4128 Datasheet / COA / SDS

Discontinued Product

MAB4128 has been discontinued.

View all CEACAM-5/CD66e products.

Detection of CEACAM‑5/CD66e in Human Granulocytes by Flow Cytometry.

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All CEACAM-5/CD66e Antibodies

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Human CEACAM-5/CD66e Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM‑5/CD66e in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CEACAM-8 is observed and no cross-reactivity with rhCEACAM-1 or rhCEACAM-6 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 487618

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM‑5/CD66e
Lys35-Ala685
Accession # ABM87752

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑5/CD66e in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB4128, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM‑5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6‑8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK‑mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14‑16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

Zebhauser, R. et al. (2005) Genomics 86:566.
Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
Garcia, M. et al. (1991) Cancer Res. 51:5679.
Stern, N. et al. (2005) J. Immunol. 174:6692.
Benchimol, S. et al. (1989) Cell 57:327.
Zhou, H. et al. (1993) J. Cell Biol. 122:951.
Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
Ordonez, C. et al. (2000) Cancer Res. 60:3419.
Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
Chen, T. et al. (1997) J. Exp. Med. 185:1557.
Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100