Human CEACAM‑5/CD66e Biotinylated Antibody

Name: Human CEACAM‑5/CD66e Biotinylated Antibody
Brand: R&D Systems
SKU: BAF4128

Catalog #: BAF4128 Datasheet / COA / SDS

Discontinued Product

BAF4128 has been discontinued.

View all CEACAM-5/CD66e products.

Detection of CEACAM‑5/CD66e in human peripheral blood granulocytes by Flow Cytometry.

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Human CEACAM‑5/CD66e Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-5 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CEACAM‑1 and rhCEACAM-6 is observed and less than 5% cross-reactivity with rhCEACAM-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM-5/CD66e
Lys35-Ala685
Accession # Q8N4D0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM)

Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑5/CD66e in human peripheral blood granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Human CEACAM-5/CD66e Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4128, filled histogram) or control antibody (Catalog # BAF020, open histogram) followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% bovine serum albumin.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6-8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14-16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

Zebhauser, R. et al. (2005) Genomics 86:566.
Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4648.
Garcia, M. et al. (1991) Cancer Res. 51:5679.
Stern, N. et al. (2005) J. Immunol. 174:6692.
Benchimol, S. et al. (1989) Cell 57:327.
Zhou, H. et al. (1993) J. Cell Biol. 122:951.
Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
Ordonez, C. et al. (2000) Cancer Res. 60:3419.
Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
Chen, T. et al. (1997) J. Exp. Med. 185:1557.
Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100