Human CEACAM‑5/CD66e Biotinylated Antibody

Catalog #: BAF4128 Datasheet / COA / SDS

Discontinued Product

BAF4128 has been discontinued.
View all CEACAM-5/CD66e products.
Detection of CEACAM‑5/CD66e in human peripheral blood granulocytes by Flow Cytometry.
1 Image
Product Details
FAQs
Reviews

Human CEACAM‑5/CD66e Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-5 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CEACAM‑1 and rhCEACAM-6 is observed and less than 5% cross-reactivity with rhCEACAM-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM-5/CD66e
Lys35-Ala685
Accession # Q8N4D0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM)
Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM-5/CD66e antibody in human peripheral blood granulocytes antibody by Flow Cytometry. View Larger

Detection of CEACAM‑5/CD66e in human peripheral blood granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Human CEACAM-5/CD66e Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4128, filled histogram) or control antibody (Catalog # BAF020, open histogram) followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% bovine serum albumin.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6-8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14-16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References
  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
  4. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4648.
  5. Garcia, M. et al. (1991) Cancer Res. 51:5679.
  6. Stern, N. et al. (2005) J. Immunol. 174:6692.
  7. Benchimol, S. et al. (1989) Cell 57:327.
  8. Zhou, H. et al. (1993) J. Cell Biol. 122:951.
  9. Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
  10. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  11. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  12. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  13. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  14. Chen, T. et al. (1997) J. Exp. Med. 185:1557.
  15. Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
  16. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Entrez Gene IDs
1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)
Alternate Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CEACAM‑5/CD66e Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human CEACAM‑5/CD66e Biotinylated Antibody and earn rewards!

Have you used Human CEACAM‑5/CD66e Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review