Human CEACAM-5/CD66e PE-conjugated Antibody

Catalog #: FAB41281P Datasheet / COA / SDS

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FAB41281P

Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry.

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Human CEACAM-5/CD66e PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM‑5/CD66e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1 or -6 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 487609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM‑5/CD66e
Lys35-Ala685
Accession # ABM87752

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) irrelevant transfetants and eGFP was stained with Mouse Anti-Human CEACAM-5/CD66e PE-conjugated Monoclonal Antibody (Catalog # FAB41281P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6-8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14-16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

Zebhauser, R. et al. (2005) Genomics 86:566.
Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
Garcia, M. et al. (1991) Cancer Res. 51:5679.
Stern, N. et al. (2005) J. Immunol. 174:6692.
Benchimol, S. et al. (1989) Cell 57:327.
Zhou, H. et al. (1993) J. Cell Biol. 122:951.
Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
Ordonez, C. et al. (2000) Cancer Res. 60:3419.
Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
Chen, T. et al. (1997) J. Exp. Med. 185:1557.
Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100