Human CEACAM-7 Biotinylated Antibody

Catalog #: BAF4478 Datasheet / COA / SDS

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BAF4478

Detection of Human CEACAM‑7 by Western Blot.

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Human CEACAM-7 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-7 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CEACAM-1, -3, -4, -5,
-6, and -8 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CEACAM‑7 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CEACAM-7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM-7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 7

Entrez Gene IDs

1087 (Human)

Alternate Names

Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEACAM-7; CGM 2; CGM2