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Human CEACAM-8/CD66b Biotinylated Antibody

Catalog #: BAF4246
1 Citation Datasheet / COA / SDS
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BAF4246
Detection of CEACAM‑8/CD66b in Human Granulocytes by Flow Cytometry.
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Human CEACAM-8/CD66b Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM‑8/CD66b in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) CEACAM-3 and rhCEACAM-7 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CEACAM‑8/CD66b
Gln35-His141
Accession # P31997
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CEACAM‑8/CD66b
Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM‑8/CD66b antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of CEACAM‑8/CD66b in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Biotinylated Sheep Anti-Human CEACAM-8/CD66b Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4246, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-8/CD66b

CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Entrez Gene IDs
1088 (Human); 102138843 (Cynomolgus Monkey)
Alternate Names
Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95

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Citation for Human CEACAM-8/CD66b Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Monitoring Neutropenia for Cancer Patients at the Point of Care
    Authors: Hakan Inan, James L. Kingsley, Mehmet O. Ozen, Huseyin Cumhur Tekin, Christian R. Hoerner, Yoriko Imae et al.
    Small Methods

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