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Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

Catalog #: DC3L10
37 Citations 4 Reviews Datasheet / COA / SDS
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DC3L10
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QC63: Quantikine Immunoassay Cntl Set 1027 Human CHI3L1/YKL-40
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Human Chitinase 3-like 1 ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
8.15 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human CHI3L1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human CHI3L1 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=36)36.015.9-93.520.6
EDTA plasma (n=36)27.710.9-79.117.5
Heparin plasma (n=36)26.810.7-72.115.7
Urine (n=11)0.640.14-1.820.49

Cell Culture Supernates:
HepG2 human hepatocellular carcinoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 0.944 ng/mL.

MG-63 human osteosarcoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate for 4 days. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 3.04 ng/mL.

U-87 MG human glioblastoma/astrocytoma cells and SK-Mel-28 human malignant melanoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 1 mM sodium pyruvate, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of each cell culture supernate was removed and assayed for human CHI3L1. U-87 MG cells measured 16.1 ng/mL, and the SK-Mel-28 cells measured 0.071 ng/mL.

HASMC human aortic smooth muscle cells were cultured in Medium 231 supplemented with smooth muscle growth supplement until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 0.623 ng/mL.

BUD-8 human fibroblasts were cultured in 90% MEM with non-essential amino acids supplemented with 10% fetal bovine serum and 2 mM L-glutamine until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 4.31 ng/mL. 

Product Summary

The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 566 1031 2202 606 1019 2212
Standard Deviation 26.7 44.4 102.6 32.1 59.1 153.1
CV% 4.7 4.3 4.7 5.3 5.8 6.9

Recovery

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 93-100
Urine (n=4) 105 90-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Chitinase 3-like 1 ELISA Linearity

Scientific Data

N/A Human Chitinase 3-like 1 ELISA Standard Curve View Larger

Human Chitinase 3-like 1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Entrez Gene IDs:
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names:
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Alzheimer's disease CSF biomarkers correlate with early pathology and alterations in neuronal and glial gene expression
    Authors: Ropri, AS;Lam, TG;Kalia, V;Buchanan, HM;Bartosch, AMW;Youth, EHH;Xiao, H;Ross, SK;Jain, A;Chakrabarty, JK;Kang, MS;Boyett, D;Spinazzi, EF;Iodice, G;McGovern, RA;Honig, LS;Brown, LM;Miller, GW;McKhann, GM;Teich, AF;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: CSF
  2. p75 neurotrophin receptor modulation in mild to moderate Alzheimer disease: a randomized, placebo-controlled phase 2a trial
    Authors: Shanks, HRC;Chen, K;Reiman, EM;Blennow, K;Cummings, JL;Massa, SM;Longo, FM;Börjesson-Hanson, A;Windisch, M;Schmitz, TW;
    Nature medicine
    Species: Human
    Sample Types: CSF
  3. Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer's Disease
    Authors: M Babi? Leko, M Mihel?i?, J Jurasovi?, M Nikolac Pe, E Špani?, A Sekovani?, T Orct, K Zub?i?, L Langer Hor, N Plei?, S Ki?emet-Pi, Ž Vogrinc, N Pivac, A Diana, F Borove?ki, PR Hof, G Šimi?
    International Journal of Molecular Sciences, 2022-12-27;24(1):.
    Species: Human
    Sample Types: CSF
  4. Plasma Human Cartilage Glycoprotein-39 Is Associated With the Prognosis of Acute Ischemic Stroke
    Authors: T Xu, K Zhang, C Zhong, Z Zhu, X Zheng, P Yang, B Che, Y Lu, Y Zhang
    Journal of the American Heart Association, 2022-09-14;0(0):e026263.
    Species: Human
    Sample Types: Serum
  5. Association of Serum GFAP with Functional and Neurocognitive Outcome in Sporadic Small Vessel Disease
    Authors: A Huss, A Abdelhak, B Mayer, H Tumani, HP Müller, K Althaus, J Kassubek, M Otto, AC Ludolph, D Yilmazer-H, H Neugebauer
    Biomedicines, 2022-08-02;10(8):.
    Species: Human
    Sample Types: CSF
  6. Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
    Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
    Scientific Reports, 2022-07-23;12(1):12606.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis
    Authors: W Francuzik, K Pažur, M Dalke, S Dölle-Bier, M Babina, M Worm
    JCI Insight, 2022-04-08;0(0):.
    Species: Human
    Sample Types: Serum
  8. Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
    Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
    Regenerative Therapy, 2021-03-27;18(0):38-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Increased plasma levels of chitinase 3-like 1 (CHI3L1) protein in patients with idiopathic normal-pressure hydrocephalus
    Authors: PW Ko, HW Lee, M Lee, YC Youn, S Kim, JH Kim, K Kang, K Suk
    Journal of the neurological sciences, 2021-02-20;423(0):117353.
    Species: Human
    Sample Types: Plasma
  10. The potential pathways underlying the association of propyl-paraben exposure with aeroallergen sensitization and EASI score using metabolomics analysis
    Authors: Y Lee, E Lee, DK Yon, HM Jee, HS Baek, SW Lee, JY Cho, MY Han
    Scientific Reports, 2021-02-12;11(1):3772.
    Species: Human
    Sample Types: Serum
  11. Cerebrospinal Fluid Biomarkers in Relation to MRZ Reaction Status in Primary Progressive Multiple Sclerosis
    Authors: T Robinson, A Abdelhak, T Bose, E Meinl, M Otto, UK Zettl, R Dersch, H Tumani, S Rauer, A Huss
    Cells, 2020-11-25;9(12):.
    Species: Human
    Sample Types: CSF
  12. Serum YKL-40 Levels Are Associated with the Atherogenic Index of Plasma in Children
    Authors: Y Kwon, JH Kim, EK Ha, HM Jee, HS Baek, MY Han, SJ Jeong
    Mediators Inflamm, 2020-09-26;2020(0):8713908.
    Species: Human
    Sample Types: Serum
  13. A Multi-Omics Approach Reveals New Signatures in Obese Allergic Asthmatic Children
    Authors: MA Gomez-Llor, A Martínez-C, N Chueca, MC Rico, R Romero, A Anguita-Ru, CM Aguilera, M Gil-Campos, MD Mesa, B Khakimov, JA Morillo, Á Gil, J Camacho, C Gomez-Llor
    Biomedicines, 2020-09-18;8(9):.
    Species: Human
    Sample Types: Plasma
  14. Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis
    Authors: G Ye, Z Xie, H Zeng, P Wang, J Li, G Zheng, S Wang, Q Cao, M Li, W Liu, S Cen, Z Li, Y Wu, Z Ye, H Shen
    Cell Death Dis, 2020-09-17;11(9):775.
    Species: Human
    Sample Types: Serum
  15. Association of glial and neuronal degeneration markers with Alzheimer's disease cerebrospinal fluid profile and cognitive functions
    Authors: UD Teitsdotti, MK Jonsdottir, SH Lund, T Darreh-Sho, J Snaedal, PH Petersen
    Alzheimers Res Ther, 2020-08-04;12(1):92.
    Species: Human
    Sample Types: CSF
  16. Combined assessment of serum eosinophil-derived neurotoxin and YKL-40 may identify Asthma-COPD overlap
    Authors: T Shirai, K Hirai, Y Gon, S Maruoka, K Mizumura, M Hikichi, K Itoh, S Hashimoto
    Allergol Int, 2020-06-20;0(0):.
    Species: Human
    Sample Types: Serum
  17. Determination of chitinase 3-like 1 in cerebrospinal fluid in multiple sclerosis and other neurological diseases
    Authors: P Kušnierová, D Zeman, P Hradílek, O Zapletalov, D Stejskal
    PLoS ONE, 2020-05-21;15(5):e0233519.
    Species: Human
    Sample Types: CSF
  18. Urinary cell cycle arrest biomarkers and chitinase 3-like protein 1 (CHI3L1) to detect acute kidney injury in the critically ill: a post hoc laboratory analysis on the FINNAKI cohort
    Authors: EA Hoste, ST Vaara, J De Loor, M Haapio, L Nuytinck, K Demeyere, V Pettilä, E Meyer
    Crit Care, 2020-04-10;24(1):144.
    Species: Human
    Sample Types: Urine
  19. Improved early detection of ovarian cancer using longitudinal multimarker models
    Authors: HJ Whitwell, J Worthingto, O Blyuss, A Gentry-Mah, A Ryan, R Gunu, J Kalsi, U Menon, I Jacobs, A Zaikin, JF Timms
    Br. J. Cancer, 2020-01-15;0(0):.
    Species: Human
    Sample Types: Serum
  20. Cerebrospinal fluid and plasma biomarker trajectories with increasing amyloid deposition in Alzheimer's disease
    Authors: S Palmqvist, PS Insel, E Stomrud, S Janelidze, H Zetterberg, B Brix, U Eichenlaub, JL Dage, X Chai, K Blennow, N Mattsson, O Hansson
    EMBO Mol Med, 2019-11-11;0(0):e11170.
    Species: Human
    Sample Types: CSF
  21. Cell-free mitochondrial DNA in progressive multiple sclerosis
    Authors: H Lowes, A Pyle, M Duddy, G Hudson
    Mitochondrion, 2018-08-08;0(0):.
    Species: Human
    Sample Types: CSF
  22. Sleep Deprivation and CSF Biomarkers for Alzheimer Disease
    Authors: M Olsson, J Ärlig, J Hedner, K Blennow, H Zetterberg
    Sleep, 2018-05-01;0(0):.
    Species: Human
    Sample Types: CSF
  23. Effects of surgery and propofol-remifentanil total intravenous anesthesia on cerebrospinal fluid biomarkers of inflammation, Alzheimer's disease, and neuronal injury in humans: a cohort study
    Authors: A Pikwer, M Castegren, S Namdar, K Blennow, H Zetterberg, N Mattsson
    J Neuroinflammation, 2017-09-29;14(1):193.
    Species: Human
    Sample Types: CSF
  24. Chitinase-3-Like Protein 1 (YKL-40) Is a New Biomarker of Inflammation in Psoriasis
    Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
    Mediators Inflamm., 2017-08-28;2017(0):9538451.
    Species: Human
    Sample Types: Serum
  25. Chitinase-3-Like Protein 1 (YKL-40) Reflects the Severity of Symptoms in Atopic Dermatitis
    Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
    J Immunol Res, 2017-06-04;2017(0):5746031.
    Species: Human
    Sample Types: Serum
  26. Effect of physical exercise on markers of neuronal dysfunction in cerebrospinal fluid in patients with Alzheimer's disease
    Authors: CS Jensen, E Portelius, P Høgh, L Wermuth, K Blennow, H Zetterberg, SG Hasselbalc, AH Simonsen
    Alzheimers Dement (N Y), 2017-04-17;3(2):284-290.
    Species: Human
    Sample Types: CSF
  27. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
    Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
    J. Clin. Invest, 2017-03-20;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Diagnosis of cardiac surgery-associated acute kidney injury: differential roles of creatinine, chitinase 3-like protein 1 and neutrophil gelatinase-associated lipocalin: a prospective cohort study
    Authors: J De Loor, I Herck, K Francois, A Van Wesema, L Nuytinck, E Meyer, EA Hoste
    Ann Intensive Care, 2017-03-01;7(1):24.
    Species: Human
    Sample Types: Urine
  29. Cerebrospinal Fluid Biomarkers of Neurodegeneration Are Decreased or Normal in Narcolepsy
    Authors: P Jørgen Jen, L Østergaard, JM Czarna Bah, S Modvig, K Fog, A Holm, B Rahbek Kor, S Gammeltoft
    Sleep, 2017-01-01;40(1):.
    Species: Human
    Sample Types: CSF
  30. Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders
    Authors: Viktoria Johansson
    Eur Arch Psychiatry Clin Neurosci, 2016-12-30;0(0):.
    Species: Human
    Sample Types: CSF
  31. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
    JCI Insight, 2016-12-08;1(20):e90954.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Least absolute shrinkage and selection operator type methods for the identification of serum biomarkers of overweight and obesity: simulation and application
    BMC Med Res Methodol, 2016-11-14;16(1):154.
    Species: Human
    Sample Types: Serum
  33. MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer
    Authors: Annette Eyking
    PLoS ONE, 2016-06-06;11(6):e0156871.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease.
    Authors: Fantino E, Gangell C, Hartl D, Sly P
    BMC Pulm Med, 2014-02-27;14(0):28.
    Species: Human
    Sample Types: BALF
  35. Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
    Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
    PLoS ONE, 2013-04-22;8(4):e61984.
    Species: Human
    Sample Types: Serum
  36. Levels of YKL-40 in pleural effusions and blood from patients with pulmonary or pleural disease.
    Authors: Kim HR, Jun CD, Lee KS, Cho JH, Jeong ET, Yang SH, Lee YJ, Park DS
    Cytokine, 2012-04-03;58(3):336-43.
    Species: Human
    Sample Types: Serum
  37. The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
    Authors: Hector A, Kormann MS, Mack I, Latzin P, Casaulta C, Kieninger E, Zhou Z, Yildirim AÖ, Bohla A, Rieber N, Kappler M, Koller B, Eber E, Eickmeier O, Zielen S, Eickelberg O, Griese M, Mall MA, Hartl D
    PLoS ONE, 2011-09-20;6(9):e24399.
    Species: Human
    Sample Types: Serum

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Reviews for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

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Human Chitinase 3-like 1 Quantikine ELISA Kit
By Anonymous on 09/12/2020
Sample Tested: Serum
Human Chitinase 3-like 1 Quantikine ELISA Kit
By Anonymous on 06/13/2019
Sample Tested: Urine
Human Chitinase 3-like 1 Quantikine ELISA Kit DC3L10
Human Chitinase 3-like 1 Quantikine ELISA Kit
By Maria Garcia on 10/15/2018
Sample Tested: EDTA Plasma
Human Chitinase 3-like 1 Quantikine ELISA Kit DC3L10
Human Chitinase 3-like 1 Quantikine ELISA Kit
By Anonymous on 08/29/2018
Sample Tested: Serum