Human Clusterin Antibody

Catalog #: MAB29373 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29373-100
MAB29373-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Clusterin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Clusterin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 350207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Clusterin
Asp23-Arg227 (beta) & Ser228-Glu449 (alpha)
Accession # NP_001822

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29372).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Clusterin DuoSet ELISA Kit (Catalog # DY5874) for convenient development of a sandwich ELISA or the Human Clusterin Quantikine ELISA Kit (Catalog # DCLU00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Clusterin ELISA Standard Curve. Recombinant Human Clusterin protein was serially diluted 2-fold and captured by Mouse Anti-Human Clusterin Monoclonal Antibody (MAB29372) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Clusterin Monoclonal Antibody (Catalog # MAB29373) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development (3). Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain (4 - 6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an 80 kDa N-glycosylated disulfide-linked heterodimer (7, 8). Mature human Clusterin shares 77% amino acid sequence identity with mouse and rat Clusterin. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (9, 10). In human, an alternately spliced 50 kDa isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular (5, 11). This molecule is neither glycosylated nor cleaved into alpha and beta chains (11). In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NF kappa B activation (12, 13). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 11). This function contrasts with the cytoprotective effect of secreted Clusterin (14). During colon cancer tumor progression there is a downregulation of the intracellular form and an upregulation of the glycosylated secreted form (11).

References

Carver, J.A. et al. (2003) IUBMB Life 55:661.
Shannan, B. et al. (2006) Cell Death Differ. 13:12.
French, L.E. et al. (1993) J. Cell Biol. 122:1119.
Kirszbaum, L. et al. (1989) EMBO J. 8:711.
Leskov, K.S. et al. (2003) J. Biol. Chem. 278:11590.
Pankhurst, G.J. et al. (1998) Biochemistry 37:4823.
Burkey, B.F. et al. (1991) J. Lipid. Res. 32:1039.
de Silva, H.V. et al. (1990) J. Biol. Chem. 265:14292.
Jenne, D.E. et al. (1991) J. Biol. Chem. 266:11030.
Kounnas, M.Z. et al. (1995) J. Biol. Chem. 270:13070.
Pucci, S. et al. (2004) Oncogene 23:2298.
Moretti, R. M. et al. (2007) Cancer Res. 67:10325.
Santilli, G. et al. (2003) J. Biol. Chem. 278:38214.
Trougakos, I.P. et al. (2004) Cancer Res. 64:1834.

Entrez Gene IDs

1191 (Human); 12759 (Mouse)

Alternate Names

40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2