Human Contactin-2/TAG1 DuoSet ELISA

Catalog #: DY1714-05
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY1714-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

Human Contactin-2 / TAG1 ELISA Standard Curve

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All Contactin-2/TAG1 ELISAs

Product Details

Citations (1)

Supplemental Products

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Contactin-2/TAG1 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

156.0 - 10,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Contactin-2 / TAG1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human Contactin-2 / TAG1 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also known as TAX1 (human), TAG1 (rat), and Axonin-1 (chicken), is a cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily. Contactin-2 contains 6 Ig-like domains followed by 4 fibronectin type III-like repeats. The gene for human CNTN2 encodes a 1040 aa precursor protein with a 28 aa signal peptide and a 984 aa mature secreted protein that is attached to the membrane via a glycosyl-phosphatidylinositol(GPI)-anchor.

Entrez Gene IDs:

6900 (Human); 21367 (Mouse)

Alternate Names:

Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

Citation for Human Contactin-2/TAG1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies
Authors: M Chatterjee, I van Steeno, E Huisman, L Oosterveld, H Berendse, WM van der Fl, M Del Campo, AW Lemstra, WDJ van de Ber, CE Teunissen
Biomolecules, 2020-08-12;10(8):.
Species: Human
Sample Types: CSF

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