Human Corneodesmosin Antibody Summary
Lys33-Pro529 (Ser153 del)
Accession # NP_001255
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Corneodesmosin in Human Skin. Corneodesmosin was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Corneodesmosin
Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).
- Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
- Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. USA 90:9470.
- Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
- Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
- Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
- Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6720.
- Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
- Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
- Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
- Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
- Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
- Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.
Product Datasheets
Citations for Human Corneodesmosin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The caspase-1 inhibitor CARD18 is specifically expressed during late differentiation of keratinocytes and its expression is lost in lichen planus
Authors: H Qin, J Jin, H Fischer, M Mildner, M Gschwandtn, V Mlitz, L Eckhart, E Tschachler
J. Dermatol. Sci., 2017-05-01;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
Authors: E Jacquin, S Leclerc-Me, C Judon, E Blanchard, S Fraitag, O Florey
Autophagy, 2017-02-15;0(0):1-14.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Callus formation is associated with hyperproliferation and incomplete differentiation of keratinocytes, and increased expression of adhesion molecules.
Authors: Kim SH, Kim S
Br. J. Dermatol., 2010-04-30;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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