Human CRTH-2 APC-conjugated Antibody

Catalog #: FAB33381A Datasheet / COA / SDS

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FAB33381A

Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry.

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Human CRTH-2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRTH‑2 in Cell-Based ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 301109

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CRTH-2
Met1-Ser395
Accession # Q9Y5Y4

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human CRTH-2 APC-conjugated Monoclonal Antibody (Catalog # FAB33381A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CRTH-2

CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Entrez Gene IDs

11251 (Human); 14764 (Mouse); 309212 (Rat)

Alternate Names

CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44