Human CXCL10/IP-10 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CXCL10/IP-10. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: CXCL10/IP-10/CRG-2
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human CXCL10/IP-10 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls
Authors: Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M;
PloS one
Species: Human
Sample Types: Vaginal Swab
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Neutrophil extracellular traps promote immunopathogenesis of virus-induced COPD exacerbations
Authors: Katsoulis, O;Toussaint, M;Jackson, MM;Mallia, P;Footitt, J;Mincham, KT;Meyer, GFM;Kebadze, T;Gilmour, A;Long, M;Aswani, AD;Snelgrove, RJ;Johnston, SL;Chalmers, JD;Singanayagam, A;
Nature communications
Species: Human
Sample Types: Sputum
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Myocardial transcriptomic analysis of diabetic patients with aortic stenosis: key role for mitochondrial calcium signaling
Authors: Cherpaz, M;Meugnier, E;Seillier, G;Pozzi, M;Pierrard, R;Leboube, S;Farhat, F;Vola, M;Obadia, JF;Amaz, C;Chalabreysse, L;May, C;Chanon, S;Brun, C;Givre, L;Bidaux, G;Mewton, N;Derumeaux, G;Bergerot, C;Paillard, M;Thibault, H;
Cardiovascular diabetology
Species: Human
Sample Types: Serum
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Octyl itaconate enhances VSV?51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways
Authors: Kurmasheva, N;Said, A;Wong, B;Kinderman, P;Han, X;Rahimic, AHF;Kress, A;Carter-Timofte, ME;Holm, E;van der Horst, D;Kollmann, CF;Liu, Z;Wang, C;Hoang, HD;Kovalenko, E;Chrysopoulou, M;Twayana, KS;Ottosen, RN;Svenningsen, EB;Begnini, F;Kiib, AE;Kromm, FEH;Weiss, HJ;Di Carlo, D;Muscolini, M;Higgins, M;van der Heijden, M;Bardoul, A;Tong, T;Ozsvar, A;Hou, WH;Schack, VR;Holm, CK;Zheng, Y;Ruzek, M;Kalucka, J;de la Vega, L;Elgaher, WAM;Korshoej, AR;Lin, R;Hiscott, J;Poulsen, TB;O'Neill, LA;Roy, DG;Rinschen, MM;van Montfoort, N;Diallo, JS;Farin, HF;Alain, T;Olagnier, D;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
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Association of the Reduced Levels of Monocyte Chemoattractant Protein-1 with Herpes Zoster in Rheumatoid Arthritis Patients Treated with Janus Kinase Inhibitors in a Single-Center Cohort
Authors: Chen, PK;Chen, YM;Chen, HH;Liao, TL;Chang, SH;Yeo, KJ;Huang, PH;Chen, DY;
Microorganisms
Species: Human
Sample Types: Plasma
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Systematic investigation of chemo-immunotherapy synergism to shift anti-PD-1 resistance in cancer
Authors: Wang, Y;Pattarayan, D;Huang, H;Zhao, Y;Li, S;Wang, Y;Zhang, M;Li, S;Yang, D;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
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Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants
Authors: Reuschl, AK;Thorne, LG;Whelan, MVX;Ragazzini, R;Furnon, W;Cowton, VM;De Lorenzo, G;Mesner, D;Turner, JLE;Dowgier, G;Bogoda, N;Bonfanti, P;Palmarini, M;Patel, AH;Jolly, C;Towers, GJ;
Nature microbiology
Species: Human
Sample Types: Cell Culture Supernates
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Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation
Authors: Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
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FBXO21 mediated degradation of p85? regulates proliferation and survival of acute myeloid leukemia
Authors: Dobish, KK;Wittorf, KJ;Swenson, SA;Bean, DC;Gavile, CM;Woods, NT;Ghosal, G;Hyde, RK;Buckley, SM;
Leukemia
Species: Human
Sample Types: Whole Cells
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High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
Authors: SMR Gomes, ACS Brito, WFP Manfro, M Ribeiro-Al, RSA Ribeiro, MS da Cal, VDC Lisboa, DPB Abreu, LDR Castilho, LCMS Porto, TT Mafort, AJ Lopes, SAG da Silva, PML Dutra, LS Rodrigues
PLoS ONE, 2023-04-05;18(4):e0283983.
Species: Human
Sample Types: Serum
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Interferon and interferon-induced cytokines as markers of impending clinical progression in ANA+ individuals without a systemic autoimmune rheumatic disease diagnosis
Authors: ST Kim, C Muñoz-Graj, SE Dunn, R Schneider, SR Johnson, Z Touma, Z Ahmad, D Bonilla, EG Atenafu, LT Hiraki, A Bookman, J Wither
Arthritis Research & Therapy, 2023-02-10;25(1):21.
Species: Human
Sample Types: Serum
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The Antimicrobial Peptide Cathelicidin Exerts Immunomodulatory Effects via Scavenger Receptors
Authors: R Amagai, T Takahashi, H Terui, T Fujimura, K Yamasaki, S Aiba, Y Asano
International Journal of Molecular Sciences, 2023-01-03;24(1):.
Species: Human
Sample Types: Cell Culture Supernates
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High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients
Authors: SMR Gomes, ACS Brito, WFP Manfro, M Ribeiro-Al, RSA Ribeiro, MS da Cal, VDC Lisboa, DPB Abreu, LDR Castilho, LCMS Porto, TT Mafort, AJ Lopes, SAG da Silva, PML Dutra, LS Rodrigues
PLoS ONE, 2023;18(4):e0283983.
Species: Human
Sample Types: Serum
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EGFR-Tyrosine Kinase Inhibitors Induced Activation of the Autocrine CXCL10/CXCR3 Pathway through Crosstalk between the Tumor and the Microenvironment in EGFR-Mutant Lung Cancer
Authors: SH Hong, N Kang, O Kim, SA Hong, J Park, J Kim, MA Lee, J Kang
Cancers, 2022-12-25;15(1):.
Species: Human
Sample Types: Cell Culture Supernates
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Genome-wide gain-of-function screening characterized lncRNA regulators for tumor immune response
Authors: Y Wang, Y Zhao, W Guo, GS Yadav, C Bhaskarla, Z Wang, X Wang, S Li, Y Wang, Y Chen, D Pattarayan, W Xie, S Li, B Lu, US Kammula, M Zhang, D Yang
Science Advances, 2022-12-07;8(49):eadd0005.
Species: Human
Sample Types: Cell Culture Supernates
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The Anti-Inflammatory Effects of Iberin on TNF-alpha-Stimulated Human Oral Epithelial Cells: In Vitro Research
Authors: Y Hosokawa, I Hosokawa, M Shimoyama, A Fujii, J Sato, K Kadena, K Ozaki, K Hosaka
Biomedicines, 2022-12-06;10(12):.
Species: Human
Sample Types: Cell Culture Supernates
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Evasion of cGAS and TRIM5 defines pandemic HIV
Authors: L Zuliani-Al, ML Govasli, J Rasaiyaah, C Monit, SO Perry, RP Sumner, S McAlpine-S, C Dickson, KM Rifat Fays, L Hilditch, RJ Miles, F Bibollet-R, BH Hahn, T Boecking, N Pinotsis, LC James, DA Jacques, GJ Towers
Nature Microbiology, 2022-10-26;7(11):1762-1776.
Species: Human
Sample Types: Cell Culture Supernates
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Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes
Authors: D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters
Scientific Reports, 2022-10-24;12(1):17827.
Species: Human
Sample Types: Cell Culture Supernates
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CXCL10 serves as a potential serum biomarker complementing SCC-Ag for diagnosing cervical squamous cell carcinoma
Authors: J Zhang, D Dong, Q Wei, L Ren
BMC Cancer, 2022-10-08;22(1):1052.
Species: Human
Sample Types: Serum
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Th1 cytokines synergize to change gene expression and promote corticosteroid insensitivity in pediatric airway smooth muscle
Authors: D Jackson, J Walum, P Banerjee, BW Lewis, YS Prakash, V Sathish, Z Xu, RD Britt
Respiratory Research, 2022-05-16;23(1):126.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells
Authors: E Filidou, L Kandilogia, G Tarapatzi, M Spathakis, P Steiropoul, D Mikroulis, K Arvanitidi, V Paspaliari, G Kolios
International Journal of Molecular Sciences, 2022-04-20;23(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Butyrate Prevents Induction of CXCL10 and Non-Canonical IRF9 Expression by Activated Human Intestinal Epithelial Cells via HDAC Inhibition
Authors: SGPJ Korsten, L Peracic, LMB van Groeni, MAP Diks, H Vromans, J Garssen, LEM Willemsen
International Journal of Molecular Sciences, 2022-04-02;23(7):.
Species: Human
Sample Types: Cell Culture Supernates
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DDX50 Is a Viral Restriction Factor That Enhances IRF3 Activation
Authors: MA Pallett, Y Lu, GL Smith
Viruses, 2022-02-03;14(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Development of Small-Molecule STING Activators for Cancer Immunotherapy
Authors: HR Jung, S Jo, MJ Jeon, H Lee, Y Chu, J Lee, E Kim, GY Song, C Jung, H Kim, S Lee
Biomedicines, 2021-12-24;10(1):.
Species: Human
Sample Types: Cell Culture Supernates
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IL-25 Induced ROS-Mediated M2 Macrophage Polarization via AMPK-Associated Mitophagy
Authors: ML Tsai, YG Tsai, YC Lin, YL Hsu, YT Chen, MK Tsai, WT Liao, YC Lin, CH Hung
International Journal of Molecular Sciences, 2021-12-21;23(1):.
Species: Human
Sample Types: Cell Culture Supernates
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Tofacitinib-Induced Modulation of Intestinal Adaptive and Innate Immunity and Factors Driving Cellular and Systemic Pharmacokinetics
Authors: B Texler, A Zollner, V Reinstadle, SJ Reider, S Macheiner, B Jelusic, A Pfister, C Watschinge, N Przysiecki, H Tilg, H Oberacher, AR Moschen
Cellular and Molecular Gastroenterology and Hepatology, 2021-10-06;0(0):.
Species: Human
Sample Types: Cell Culture Super
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis
Authors: L Kandilogia, E Filidou, I Drygiannak, G Tarapatzi, S Didaskalou, M Koffa, K Arvanitidi, G Bamias, V Valatas, V Paspaliari, G Kolios
Stem Cells International, 2021-07-27;2021(0):9929461.
Species: Human
Sample Types: Cell Culture Supernates
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Nobiletin Decreases Inflammatory Mediator Expression in Tumor Necrosis Factor-Stimulated Human Periodontal Ligament Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki
Mediators of Inflammation, 2021-07-10;2021(0):5535844.
Species: Human
Sample Types: Cell Culture Supernates
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Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
Scientific Reports, 2021-06-23;11(1):13100.
Species: Human
Sample Types: Serum
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Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration
Authors: HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland
Frontiers in Pharmacology, 2021-05-13;12(0):679741.
Species: Human
Sample Types: Cell Culture Supernates
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Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
Biomolecules, 2021-02-21;11(2):.
Species: Human
Sample Types: Plasma
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The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1&beta-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
BioMed Research International, 2021-01-28;2021(0):8826586.
Species: Human
Sample Types: Cell Culture Supernates
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Magnitude and timing of the antiviral response determine SARS-CoV-2 replication early in infection
Authors: NR Cheemarla, TA Watkins, VT Mihaylova, B Wang, D Zhao, G Wang, ML Landry, EF Foxman
medRxiv : the preprint server for health sciences, 2021-01-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses
Authors: H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela
Scientific Reports, 2021-01-21;11(1):1949.
Species: Human
Sample Types: Cell Culture Supernates
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The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans
Authors: S Agarwal, T Vierbuchen, S Ghosh, J Chan, Z Jiang, RK Kandasamy, E Ricci, KA Fitzgerald
Nature Communications, 2020-12-11;11(1):6348.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
Frontiers in Pharmacology, 2020-12-08;11(0):598702.
Species: Human
Sample Types: Cell Culture Supernates
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Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection
Authors: S Novelli, C Lécuroux, C Goujard, J Reynes, A Villemant, L Blum, A Essat, V Avettand-F, O Launay, JM Molina, C Bourgeois, L Meyer
EBioMedicine, 2020-11-26;62(0):103129.
Species: Human
Sample Types: Plasma
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CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features
Authors: M Kameda, M Otsuka, H Chiba, K Kuronuma, T Hasegawa, H Takahashi, H Takahashi
PLoS ONE, 2020-11-02;15(11):e0241719.
Species: Human
Sample Types: Serum
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Interleukin-18 and Hematopoietic Recovery after Allogeneic Stem Cell Transplantation
Authors: A Radujkovic, L Kordelas, R Bogdanov, C Müller-Tid, DW Beelen, P Dreger, T Luft
Cancers (Basel), 2020-09-28;12(10):.
Species: Human
Sample Types: Serum
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Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
PLoS Pathog., 2020-08-18;16(8):e1008781.
Species: Human
Sample Types: Cell Culture Supernates
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Reprogramming of profibrotic macrophages for treatment of bleomycin-induced pulmonary fibrosis
Authors: F Zhang, EA Ayaub, B Wang, E Puchulu-Ca, YH Li, SU Hettiarach, SD Lindeman, Q Luo, S Rout, M Srinivasar, A Cox, K Tsoyi, C Nickerson-, IO Rosas, PS Low
EMBO Mol Med, 2020-06-29;0(0):e12034.
Species: Human
Sample Types: Cell Culture Supernates
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TLR3 expression by maternal and fetal cells at the maternal-fetal interface in normal and preeclamptic pregnancies
Authors: LM Gierman, GB Silva, Z Pervaiz, JJ Rakner, SB Mundal, AJ Thaning, I Nervik, M Elschot, S Mathew, LCV Thomsen, L Bjørge, AC Iversen
J. Leukoc. Biol., 2020-06-23;0(0):.
Species: Human
Sample Types: Placental Supernates
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Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions
Authors: ZS Wong, SL Sokol-Borr, P Olias, JP Dubey, JP Boyle
PLoS Pathog., 2020-06-23;16(6):e1008528.
Species: Human
Sample Types: Cell Culture Supernates
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Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion
Authors: T Zhuang, X Yi, J Chen, P Kang, X Chen, J Chen, T Cui, Y Chang, Z Ye, Q Ni, Y Wang, P Du, B Li, L Liu, Z Jian, K Li, T Gao, S Li, C Li
Cell Death Dis, 2020-06-12;11(6):453.
Species: Human
Sample Types: Cell Culture Supernates
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Interleukin (IL)-17/IL-36 axis participates to the crosstalk between endothelial cells and keratinocytes during inflammatory skin responses
Authors: L Mercurio, CM Failla, L Capriotti, C Scarponi, F Facchiano, M Morelli, S Rossi, G Pagnanelli, C Albanesi, A Cavani, S Madonna
PLoS ONE, 2020-04-30;15(4):e0222969.
Species: Human
Sample Types: Cell Culture Supernates
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Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
J Immunol Res, 2020-04-21;2020(0):8074183.
Species: Human
Sample Types: Cell Culture Supernates, Plasma
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Human Cytomegalovirus (HCMV) Reactivation in the Mammary Gland Induces a Proinflammatory Cytokine Shift in Breast Milk
Authors: T Rabe, K Lazar, C Cambronero, R Goelz, K Hamprecht
Microorganisms, 2020-02-20;8(2):.
Species: Human
Sample Types: Breast Milk
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Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells
Authors: JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally
Cell Death Dis, 2020-01-27;11(1):68.
Species: Human
Sample Types: Cell Culture Supernates
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Changes in monocyte subsets are associated with clinical outcomes in severe malarial anaemia and cerebral malaria
Authors: J Royo, M Rahabi, C Kamaliddin, S Ezinmegnon, D Olagnier, H Authier, A Massougbod, J Alao, Y Ladipo, P Deloron, G Bertin, B Pipy, A Coste, A Aubouy
Sci Rep, 2019-11-26;9(1):17545.
Species: Human
Sample Types: Plasma
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Increased frequency of activated CD8+ T cell effectors in patients with psoriatic arthritis
Authors: M Diani, F Casciano, L Marongiu, M Longhi, A Altomare, PD Pigatto, P Secchiero, R Gambari, G Banfi, AA Manfredi, G Altomare, F Granucci, E Reali
Sci Rep, 2019-07-26;9(1):10870.
Species: Human
Sample Types: Serum
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Interleukin 1 Receptor-Like 1 (IL1RL1) Promotes Airway Bacterial and Viral Infection and Inflammation
Authors: N Schaunaman, A Sanchez, KG Dimasuay, N Pavelka, M Numata, R Alam, RJ Martin, HW Chu
Infect. Immun., 2019-06-20;87(7):.
Species: Human
Sample Types: Cell Culture Supernates
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The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
Sci Rep, 2019-06-06;9(1):8348.
Species: Human
Sample Types: Cell Culture Supernates
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Historical BCG vaccination combined with drug treatment enhances inhibition of mycobacterial growth ex vivo in human peripheral blood cells
Authors: SA Prabowo, A Zelmer, L Stockdale, U Ojha, SG Smith, K Seifert, HA Fletcher
Sci Rep, 2019-03-19;9(1):4842.
Species: Human
Sample Types: Cell Culture Supernates
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Phenotypic and functional stability of leukocytes from human peripheral blood samples: considerations for the design of immunological studies
Authors: A Navas, L Giraldo-Pa, MD Prieto, J Cabrera, MA Gómez
BMC Immunol., 2019-01-18;20(1):5.
Species: Human
Sample Types: Cell Culture Supernates
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An Anti-Inflammatory Azaphenothiazine Inhibits Interferon ? Expression and CXCL10 Production in KERTr Cells
Authors: L Strzadala, A Fiedorowic, E Wysokinska, E Ziolo, M Grudzie?, M Jelen, K Pluta, B Morak-Mlod, M Zimecki, W Kalas
Molecules, 2018-09-24;23(10):.
Species: Human
Sample Types: Cell Culture Supernates
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Application of Venn's diagram in the diagnosis of pleural tuberculosis using IFN-?, IP-10 and adenosine deaminase
Authors: AP Santos, RDS Corrêa, M Ribeiro-Al, ACO Soares da, TT Mafort, J Leung, GMB Pereira, LS Rodrigues, R Rufino
PLoS ONE, 2018-08-27;13(8):e0202481.
Species: Human
Sample Types: Whole Cells
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CD46 activation induces distinct CXCL-10 response in monocytes and monocyte-derived dendritic cells
Authors: AS Hansen, M Biltoft, B Bundgaard, AB Bohn, BK Møller, P Höllsberg
Cytokine, 2018-07-04;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Comparison of pro- and anti-inflammatory responses in paired human primary airway epithelial cells and alveolar macrophages
Authors: RA Mubarak, N Roberts, RJ Mason, S Alper, HW Chu
Respir. Res., 2018-06-25;19(1):126.
Species: Human
Sample Types: Cell Culture Supernates
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Direct antiviral properties of TLR ligands against HBV replication in immune-competent hepatocytes
Authors: J Lucifora, M Bonnin, L Aillot, F Fusil, S Maadadi, L Dimier, M Michelet, O Floriot, A Ollivier, M Rivoire, M Ait-Gougho, S Daffis, SP Fletcher, A Salvetti, FL Cosset, F Zoulim, D Durantel
Sci Rep, 2018-03-29;8(1):5390.
Species: Human
Sample Types: Cell Culture Supernates
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Interaction between Toll-Like Receptor 9-CpG Oligodeoxynucleotides and Hepatitis B Virus Virions Leads to Entry Inhibition in Hepatocytes and Reduction of Alpha Interferon Production by Plasmacytoid Dendritic Cells
Authors: L Aillot, M Bonnin, M Ait-Gougho, N Bendriss-V, S Maadadi, L Dimier, M Subic, C Scholtes, I Najera, F Zoulim, J Lucifora, D Durantel
Antimicrob. Agents Chemother., 2018-03-27;62(4):.
Species: Human
Sample Types: Cell Culture Supernates
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Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1
Authors: T Prabakaran, C Bodda, C Krapp, BC Zhang, MH Christense, C Sun, L Reinert, Y Cai, SB Jensen, MK Skouboe, JR Nyengaard, CB Thompson, RJ Lebbink, GC Sen, G van Loo, R Nielsen, M Komatsu, LN Nejsum, MR Jakobsen, M Gyrd-Hanse, SR Paludan
EMBO J., 2018-03-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Gasoline particle filter reduces oxidative DNA damage in bronchial epithelial cells after whole gasoline exhaust exposure in vitro
Authors: J Usemann, M Roth, C Bisig, P Comte, J Czerwinski, ACR Mayer, P Latzin, L Müller
Sci Rep, 2018-02-02;8(1):2297.
Species: Human
Sample Types: Cell Culture Supernates
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IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206.
Species: Human
Sample Types: Cell Culture Supernates
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Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
J. Biol. Chem., 2017-07-17;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE)
Authors: V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso
Arthritis Res. Ther., 2017-06-15;19(1):139.
Species: Human
Sample Types: Serum
-
CXCR3 mediates ascites-directed tumor cell migration and predicts poor outcome in ovarian cancer patients
Authors: C Windmüller, D Zech, S Avril, M Boxberg, T Dawidek, B Schmalfeld, M Schmitt, M Kiechle, H Bronger
Oncogenesis, 2017-05-15;6(5):e331.
Species: Human
Sample Types: Cell Lysates
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Excess atherosclerosis in systemic lupus erythematosus,-A matter of renal involvement: Case control study of 281 SLE patients and 281 individually matched population controls
Authors: JT Gustafsson, M Herlitz Li, I Gunnarsson, S Pettersson, K Elvin, J Öhrvik, A Larsson, K Jensen-Urs, E Svenungsso
PLoS ONE, 2017-04-17;12(4):e0174572.
Species: Human
Sample Types: Tissue Homogenates
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Identification of an immunogenic neo-epitope encoded by mouse sarcoma using CXCR3 ligand mRNAs as sensors
Authors: K Fujii, Y Miyahara, N Harada, D Muraoka, M Komura, R Yamaguchi, H Yagita, J Nakamura, S Sugino, S Okumura, S Imoto, S Miyano, H Shiku
Oncoimmunology, 2017-03-20;6(5):e1306617.
Species: Human
Sample Types: Cell Culture Supernates
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Suction blistering the lesional skin of vitiligo patients reveals useful biomarkers of disease activity
Authors: JP Strassner, M Rashighi, M Ahmed Refa, JM Richmond, JE Harris
J. Am. Acad. Dermatol, 2017-03-01;0(0):.
Species: Human
Sample Types: Blister Fluid
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IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes
Authors: JF Almine, CA O'Hare, G Dunphy, IR Haga, RJ Naik, A Atrih, DJ Connolly, J Taylor, IR Kelsall, AG Bowie, PM Beard, L Unterholzn
Nat Commun, 2017-02-13;8(0):14392.
Species: Human
Sample Types: Cell Culture Supernates
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Modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide
Sci Rep, 2016-11-14;6(0):36575.
Species: Human
Sample Types: Cell Culture Supernates
-
DUSP1 maintains IRF1 and leads to increased expression of IRF1-dependent genes: A mechanism promoting glucocorticoid-insensitivity
J Biol Chem, 2016-08-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
CXCL9 and CXCL10 predict survival and are regulated by cyclooxygenase inhibition in advanced serous ovarian cancer
Br J Cancer, 2016-08-04;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
The MNK-1/eIF4E pathway as a new therapeutic pathway to target inflammation and remodelling in asthma
Cell Signal, 2016-07-11;28(10):1555-1562.
Species: Human
Sample Types: Cell Culture Supernates
-
PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer.
Authors: Nagarsheth N, Peng D, Kryczek I, Wu K, Li W, Zhao E, Zhao L, Wei S, Frankel T, Vatan L, Szeliga W, Dou Y, Owens S, Marquez V, Tao K, Huang E, Wang G, Zou W
Cancer Res, 2015-11-13;76(2):275-82.
Species: Human
Sample Types: Cell Culture Supernates
-
Fingolimod induces neuroprotective factors in human astrocytes.
Authors: Hoffmann F, Hofereiter J, Rubsamen H, Melms J, Schwarz S, Faber H, Weber P, Putz B, Loleit V, Weber F, Hohlfeld R, Meinl E, Krumbholz M
J Neuroinflammation, 2015-09-30;12(0):184.
Species: Human
Sample Types: Cell Lysates
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Effect of JAK Inhibitors on Release of CXCL9, CXCL10 and CXCL11 from Human Airway Epithelial Cells.
Authors: Fenwick P, Macedo P, Kilty I, Barnes P, Donnelly L
PLoS ONE, 2015-06-19;10(6):e0128757.
Species: Human
Sample Types: Cell Culture Supernates
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Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
Authors: Jaguin M, Fardel O, Lecureur V
PLoS ONE, 2015-02-24;10(2):e0116560.
Species: Human
Sample Types: Cell Culture Supernates
-
Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
Nat Commun, 2014-06-09;5(0):4092.
Species: Human
Sample Types: Cell Culture Supernates
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Distinct immune response in two MERS-CoV-infected patients: can we go from bench to bedside?
Authors: Faure E, Poissy J, Goffard A, Fournier C, Kipnis E, Titecat M, Bortolotti P, Martinez L, Dubucquoi S, Dessein R, Gosset P, Mathieu D, Guery B
PLoS ONE, 2014-02-14;9(2):e88716.
Species: Human
Sample Types: Serum
-
Lipopolysaccharide engineering in Neisseria meningitidis: structural analysis of different pentaacyl lipid A mutants and comparison of their modified agonist properties.
Authors: Pupo E, Hamstra H, Meiring H, van der Ley P
J Biol Chem, 2014-02-03;289(12):8668-80.
Species: Human
Sample Types: Cell Culture Supernates
-
Inflammatory cytokines break down intrinsic immunological tolerance of human primary keratinocytes to cytosolic DNA.
Authors: Chiliveru S, Rahbek S, Jensen S, Jorgensen S, Nissen S, Christiansen S, Mogensen T, Jakobsen M, Iversen L, Johansen C, Paludan S
J Immunol, 2014-01-31;192(5):2395-404.
Species: Human
Sample Types: Cell Culture Supernates
-
Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages.
Authors: Zughaier, Susu M, Kandler, Justin L, Shafer, William
PLoS ONE, 2014-01-28;9(1):e87688.
Species: Human
Sample Types: Cell Culture Supernates
-
Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014-01-20;14(0):35.
Species: Human
Sample Types: Serum
-
Decidual natural killer cell interactions with trophoblasts are impaired in pregnancies at increased risk of preeclampsia.
Authors: Wallace A, Host A, Whitley G, Cartwright J
Am J Pathol, 2013-10-06;183(6):1853-61.
Species: Human
Sample Types: Cell Culture Supernates
-
Functional OCT4-specific CD4 and CD8 T cells in healthy controls and ovarian cancer patients.
Authors: Di J, Massuger L, Duiveman-de Boer T, Zusterzeel P, Figdor C, Torensma R
Oncoimmunology, 2013-04-01;2(5):e24271.
Species: Human
Sample Types: Cell Culture Supernates
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CXCR3 axis in patients with primary biliary cirrhosis: a possible novel mechanism of the effect of ursodeoxycholic acid.
Authors: Manousou P, Kolios G, Drygiannakis I, Koulentaki M, Pyrovolaki K, Voumvouraki A, Notas G, Bourikas L, Papadaki H, Kouroumalis E
Clin Exp Immunol, 2013-04-01;172(1):9-15.
Species: Human
Sample Types: Serum
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Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
Hum Mol Genet, 2013-01-31;22(9):1807-15.
Species: Human
Sample Types: Serum
-
Age-related changes in expression and function of Toll-like receptors in human skin.
Authors: Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, Schoppl A, Tschachler E, Elbe-Burger A
Development, 2012-10-03;139(22):4210-9.
Species: Human
Sample Types: Cell Culture Supernates
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Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
Authors: Suh HS, Choi N, Tarassishin L, Lee SC
PLoS ONE, 2012-04-11;7(4):e35115.
Species: Human
Sample Types: Cell Culture Supernates
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Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae.
Authors: Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P
J. Immunol., 2011-03-02;186(7):3966-76.
Species: Human
Sample Types: Cell Culture Supernates
-
Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
PLoS ONE, 2011-02-25;6(2):e17440.
Species: Human
Sample Types: Plasma
-
Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
Clinics (Sao Paulo), 2011-01-01;66(10):1699-705.
Species: Human
Sample Types: Plasma
-
Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010-12-29;5(12):e15291.
Species: Human
Sample Types: Plasma
-
Role of double-stranded RNA pattern recognition receptors in rhinovirus-induced airway epithelial cell responses.
Authors: Wang Q, Nagarkar DR, Bowman ER, Schneider D, Gosangi B, Lei J, Zhao Y, McHenry CL, Burgens RV, Miller DJ, Sajjan U, Hershenson MB
J. Immunol., 2009-11-04;183(11):6989-97.
Species: Human
Sample Types: Cell Culture Supernates
-
Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
Brain Behav. Immun., 2009-05-04;23(8):1079-82.
Species: Human
Sample Types: Serum
-
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation, 2009-03-02;119(10):1424-32.
Species: Human
Sample Types: Plasma
-
Hyperexpression of NOD2 in intestinal mast cells of Crohn's disease patients: preferential expression of inflammatory cell-recruiting molecules via NOD2 in mast cells.
Authors: Okumura S, Yuki K, Kobayashi R, Okamura S, Ohmori K, Saito H, Ra C, Okayama Y
Clin. Immunol., 2008-10-19;130(2):175-85.
Species: Human
Sample Types: Cell Culture Supernates
-
Divergent effects of hypoxia on dendritic cell functions.
Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
Blood, 2008-08-11;112(9):3723-34.
Species: Human
Sample Types: Cell Culture Supernates
-
Vitamin D and glucocorticoids differentially modulate chemokine expression in human airway smooth muscle cells.
Authors: Banerjee A, Damera G, Bhandare R, Gu S, Lopez-Boado Y, Panettieri R, Tliba O
Br. J. Pharmacol., 2008-06-16;155(1):84-92.
Species: Human
Sample Types: Cell Culture Supernates
-
Adrenomedullin suppresses tumour necrosis factor alpha-induced CXC chemokine ligand 10 production by human gingival fibroblasts.
Authors: Hosokawa I, Hosokawa Y, Ozaki K, Nakae H, Matsuo T
Clin. Exp. Immunol., 2008-04-24;152(3):568-75.
Species: Human
Sample Types: Cell Culture Supernates
-
Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
FASEB J., 2008-03-25;22(8):2747-57.
Species: Human
Sample Types: Cell Culture Supernates
-
Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
Authors: Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
Thorax, 2008-02-14;63(9):810-6.
Species: Human
Sample Types: Serum
-
Increased serum levels of CCL11/eotaxin in schizophrenia.
Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4.
Species: Human
Sample Types: Serum
-
The role of IkappaB kinase 2, but not activation of NF-kappaB, in the release of CXCR3 ligands from IFN-gamma-stimulated human bronchial epithelial cells.
Authors: Tudhope SJ, Catley MC, Fenwick PS, Russell RE, Rumsey WL, Newton R, Barnes PJ, Donnelly LE
J. Immunol., 2007-11-01;179(9):6237-45.
Species: Human
Sample Types: Cell Culture Supernates
-
Respiratory syncytial virus synergizes with Th2 cytokines to induce optimal levels of TARC/CCL17.
Authors: Monick MM, Powers LS, Hassan I, Groskreutz D, Yarovinsky TO, Barrett CW, Castilow EM, Tifrea D, Varga SM, Hunninghake GW
J. Immunol., 2007-08-01;179(3):1648-58.
Species: Human
Sample Types: Cell Culture Supernates
-
Distinct cytokine-driven responses of activated blood gammadelta T cells: insights into unconventional T cell pleiotropy.
Authors: Vermijlen D, Ellis P, Langford C, Klein A, Engel R, Willimann K, Jomaa H, Hayday AC, Eberl M
J. Immunol., 2007-04-01;178(7):4304-14.
Species: Human
Sample Types: Cell Culture Supernates
-
Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
Authors: Walker J, Tough DF
Eur. J. Immunol., 2006-07-01;36(7):1827-36.
Species: Human
Sample Types: Cell Culture Supernates
-
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
Authors: LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labeta MO
J. Immunol., 2006-03-15;176(6):3742-52.
Species: Human
Sample Types: Cell Culture Supernates
-
CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
Am. J. Pathol., 2005-09-01;167(3):887-99.
Species: Human
Sample Types: Cell Culture Supernates
-
Prediction of acute renal allograft rejection by urinary monokine induced by IFN-gamma (MIG).
Authors: Hauser IA, Spiegler S, Kiss E, Gauer S, Sichler O, Scheuermann EH, Ackermann H, Pfeilschifter JM, Geiger H, Grone HJ, Radeke HH
J. Am. Soc. Nephrol., 2005-04-27;16(6):1849-58.
Species: Human
Sample Types: Urine
-
The cytokine IL-1beta activates IFN response factor 3 in human fetal astrocytes in culture.
Authors: Rivieccio MA, John GR, Song X, Suh HS, Zhao Y, Lee SC, Brosnan CF
J. Immunol., 2005-03-15;174(6):3719-26.
Species: Human
Sample Types: Cell Culture Supernates
-
Chemokines and chemokine receptors in inflammatory demyelinating neuropathies: a central role for IP-10.
Authors: Kieseier BC, #N*´)2ZP, Tani M, y§/ÔcØÞ, Mahad D, Oka N, Ho T, Woodroofe N, Griffin JW, Toyka KV, Ransohoff RM, Hartung HP
2002-04-01;125(0):823-34.
Species: Human
Sample Types: CSF
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Used in Serum and EDTA Plasma neat, some samples gave high %CV.