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Human CXCL9/MIG ELISA Kit - Quantikine

Catalog #: DCX900
33 Citations 3 Reviews Datasheet / COA / SDS
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DCX900
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QC123: Quantikine Immunoassay Control Set 860 for Human CXCL9/MIG
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Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve
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Citations (33)
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Reviews (3)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CXCL9/MIG Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
11.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human MIG in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=40)64.465ND-199
EDTA plasma (n=23)56.762ND-179
Heparin plasma (n=25)62.068ND-209
ND=Non-detectable

Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of natural human MIG.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
UnstimulatedND445
Stimulated77.512,290
ND=Non-detectable


Product Summary

The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 235 700 1358 242 707 1391
Standard Deviation 9.5 25.1 24.3 20.8 50.9 83.6
CV% 4 3.6 1.8 8.6 7.2 6

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 253 817 1603 245 723 1427
Standard Deviation 9.9 26.6 49.6 12.8 59.4 88.1
CV% 3.9 3.3 3.1 5.2 8.2 6.2

Recovery

The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 86-98
EDTA Plasma (n=5) 100 95-110
Heparin Plasma (n=5) 109 101-114
Serum (n=5) 108 101-113

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of MIG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL9/MIG ELISA Linearity

Scientific Data

N/A Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve View Larger

Human CXCL9/MIG ELISA Cell Culture Supernate Standard Curve

N/A Human CXCL9/MIG ELISA Serum/Plasma Standard Curve View Larger

Human CXCL9/MIG ELISA Serum/Plasma Standard Curve

N/A Human CXCL9/MIG Quantikine ELISA Kit View Larger

Human CXCL9/MIG Quantikine ELISA Kit Serum ELISA results of three protein biomarkers in renal transplantation.We measured the protein concentration of ten genes by ELISA in independent serum samples of 19 AR patients and 20 patients with stable (STA) graft function after renal transplant. The protein concentrations of PECAM1 (A), CXCL9 (B), and CD44 (C) were higher in the AR serum samples, as shown in the notched boxplots. When the notches about two medians do not overlap, the medians are roughly significantly different at about a 95% confidence level [54]. The circles are outliers. P-values were calculated using the Mann-Whitney U test. One of the AR samples was inadvertently lost during the PECAM1 experiment and could not be recovered. (D) Areas under the Receiver Operating Characteristic (ROC) curves used to distinguish AR from STA were 0.811, 0.864, and 0.761 for PECAM1, CXCL9, and CD44, respectively. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pcbi.1000940), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Entrez Gene IDs:
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL9/MIG Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Pansclerotic morphea is characterized by IFN-gamma responses priming dendritic cell fibroblast crosstalk to promote fibrosis
    Authors: Xing, E;Ma, F;Wasikowski, R;Billi, AC;Gharaee-Kermani, M;Fox, J;Dobry, C;Victory, A;Sarkar, M;Xing, X;Plazyo, O;Chen, HW;Barber, GC;Jacobe, H;Tsou, PS;Modlin, RL;Varga, J;Kahlenberg, JM;Tsoi, LC;Gudjonsson, JE;Khanna, D;
    JCI insight
    Species: Human
    Sample Types: Serum
  2. Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
    Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
    Nature Microbiology, 2022-12-15;0(0):.
    Species: Human
    Sample Types: Serum
  3. Dynamics of Inflammatory and Neurodegenerative Biomarkers after Autologous Hematopoietic Stem Cell Transplantation in Multiple Sclerosis
    Authors: J Ruder, G Dinner, A Maceski, E Berenjeno-, AM Müller, I Jelcic, J Kuhle, R Martin
    International Journal of Molecular Sciences, 2022-09-19;23(18):.
    Species: Human
    Sample Types: Serum
  4. Interferon-gamma-Inducible Chemokines as Prognostic Markers for Lung Cancer
    Authors: KS Lee, WY Chung, JE Park, YJ Jung, JH Park, SS Sheen, KJ Park
    International Journal of Environmental Research and Public Health, 2021-09-04;18(17):.
    Species: Human
    Sample Types: Serum
  5. Murlentamab, a Low Fucosylated Anti-M�llerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation
    Authors: M Prat, M Salon, T Allain, O Dubreuil, G Noël, L Preisser, B Jean, L Cassard, F Lemée, I Tabah-Fish, B Pipy, P Jeannin, JF Prost, JM Barret, A Coste
    Cancers, 2021-04-13;13(8):.
    Species: Human
    Sample Types: Cell Lysates
  6. Nanoparticle-Mediated Delivery of 2-Deoxy-D-Glucose Induces Antitumor Immunity and Cytotoxicity in Liver Tumors in Mice
    Authors: K Sasaki, S Nishina, A Yamauchi, K Fukuda, Y Hara, M Yamamura, K Egashira, K Hino
    Cell Mol Gastroenterol Hepatol, 2020-10-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Interleukin-1? induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration
    Authors: YC Guo, YH Chiu, CP Chen, HS Wang
    Stem Cell Res Ther, 2018-10-25;9(1):281.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. EGFR signaling suppresses type 1 cytokine-induced T-cell attracting chemokine secretion in head and neck cancer
    Authors: W Ma, F Concha-Ben, SJAM Santegoets, MJP Welters, I Ehsan, RL Ferris, SH van der Bu
    PLoS ONE, 2018-09-07;13(9):e0203402.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017-05-03;6(5):.
    Species: Human
    Sample Types: Plasma
  10. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017-04-20;7(0):46580.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
    Authors: Altara R, Gu Y, Struijker-Boudier H, Thijs L, Staessen J, Blankesteijn W
    PLoS ONE, 2015-10-27;10(10):e0141394.
    Species: Human
    Sample Types: Plasma
  12. Serum CXCL9 levels are associated with tumor progression and treatment outcome in patients with nasopharyngeal carcinoma.
    Authors: Hsin L, Kao H, Chen I, Tsang N, Hsu C, Liu S, Chang Y, Chang K
    PLoS ONE, 2013-11-21;8(11):e80052.
    Species: Human
    Sample Types: Serum
  13. Increased CXCL9 serum levels in hepatitis C-related mixed cryoglobulinemia, with autoimmune thyroiditis, associated with high levels of CXCL10.
    Authors: Antonelli A, Fallahi P, Ferrari S, Colaci M, Giuggioli D, Saraceno G, Benvenga S, Ferri C
    J Interferon Cytokine Res, 2013-07-31;33(12):739-45.
    Species: Human
    Sample Types: Serum
  14. Expression of CXCR3 and its ligands CXCL9, -10 and -11 in paediatric opsoclonus-myoclonus syndrome.
    Authors: Pranzatelli M, Tate E, McGee N, Travelstead A, Verhulst S, Ransohoff R
    Clin Exp Immunol, 2013-06-01;172(3):427-36.
    Species: Human
    Sample Types: Serum
  15. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013-01-16;10(0):10.
    Species: Human
    Sample Types: Serum
  16. Circulating chemokine (CXC motif) ligand (CXCL)9 is increased in aggressive chronic autoimmune thyroiditis, in association with CXCL10.
    Authors: Antonelli A, Ferrari SM, Frascerra S, Galetta F, Franzoni F, Corrado A, Miccoli M, Benvenga S, Paolicchi A, Ferrannini E, Fallahi P
    Cytokine, 2011-05-20;55(2):288-93.
    Species: Human
    Sample Types: Serum
  17. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011-04-14;7(4):e1002016.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2009-10-22;51(2):643-50.
    Species: Human
    Sample Types: Tears
  19. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009-07-22;2009(0):269541.
    Species: Human
    Sample Types: Serum
  20. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009-04-22;34(3):676-86.
    Species: Human
    Sample Types: BALF
  21. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009-03-15;182(6):3919-27.
    Species: Human
    Sample Types: Plasma
  22. Urinary fractalkine is a marker of acute rejection.
    Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
    Kidney Int., 2008-09-17;74(11):1454-60.
    Species: Human
    Sample Types: Urine
  23. The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
    Authors: Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egesten A
    J. Interferon Cytokine Res., 2008-03-01;28(3):191-6.
    Species: Human
    Sample Types: Seminal Plasma
  24. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
    Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
    Am. J. Respir. Crit. Care Med., 2007-10-18;177(1):44-55.
    Species: Human
    Sample Types: BALF
  25. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007-10-09;133(1):26-33.
    Species: Human
    Sample Types: Saliva
  26. Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection.
    Authors: Clay CC, Rodrigues DS, Ho YS, Fallert BA, Janatpour K, Reinhart TA, Esser U
    J. Virol., 2007-08-22;81(21):12040-8.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  27. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
    Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
    Am. J. Respir. Cell Mol. Biol., 2007-08-20;38(2):168-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
    Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
    Clin. Exp. Immunol., 2007-03-01;147(3):483-90.
    Species: Human
    Sample Types: Serum
  29. Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells.
    Authors: Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P
    Cytokine, 2007-02-09;36(5):211-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
    Authors: Kanda N, Watanabe S
    Endocrinology, 2007-01-25;148(5):2317-25.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
    Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
    J. Immunol., 2007-01-15;178(2):1172-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
    Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
    Chest, 2006-10-01;130(4):982-8.
    Species: Human
    Sample Types: BALF
  33. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004-11-15;78(9):1341-50.
    Species: Human
    Sample Types: Urine

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Reviews for Human CXCL9/MIG Quantikine ELISA Kit

Average Rating: 5 (Based on 3 Reviews)

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Human CXCL9/MIG Quantikine ELISA Kit
By Mike Ehrhardt on 11/24/2020
Sample Tested: EDTA Plasma
Human CXCL9/MIG Quantikine ELISA Kit DCX900
Human CXCL9/MIG Quantikine ELISA Kit
By Anonymous on 11/09/2020
Sample Tested: Peripheral blood mononuclear cells (PBMCs)

PBMCs from healthy donors stimulated with IFNg for 16h. Samples diluted 1:2.

Human CXCL9/MIG Quantikine ELISA Kit DCX900
Human CXCL9/MIG Quantikine ELISA Kit
By Anonymous on 11/07/2019
Sample Tested: Cell culture supernatant
Human CXCL9/MIG Quantikine ELISA Kit DCX900