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Human CXCR4 Antibody

Catalog #: MAB173
29 Citations Datasheet / COA / SDS
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MAB173-100
MAB173-SP
Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody.
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Citations (29)
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Human CXCR4 Antibody Summary

Species Reactivity
Human
Specificity
Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.
Source
Monoclonal Mouse IgG2B Clone # 44717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
3T3 cells transfected with human CXCR4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Jurkat human acute T cell leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. View Larger

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND50 is typically 1-5 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of Canine CXCR4 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Canine CXCR4 by Immunocytochemistry/Immunofluorescence Ectopic expression of CXCR7 and CXCR4 on MDCK cells.(A) MDCK were stably transfected with empty vector (Mock), with CXCR7, CXCR4, a vector coding for a CXCR7 lacking the cytoplasmic C-terminus ( delta CXCR7), and a vector coding for a chimeric CXCR7 containing the DRYLAIV motive of CXCR4. Receptor expression was determined by FACS analysis using saturating antibody concentrations (see Methods). (B) Confocal immunofluorescence analysis of unfixed MDCK cells expressing CXCR7 (upper panels) or CXCR4 (lower panels). Cells also expressed N-ter-Lck mCherry as membrane marker (red fluorescence). Left panels: confocal images of planes cut through intracellular regions of MDCK monolayers. Right panels: x-z planes reconstructed from confocal x-y stacks. For receptor (green) detection anti-CXCR7 (11G8 R&D) or anti CXCR4 (MAB173 R&D) were used. Receptor-bound primary antibodies were revealed with goat anti mouse IgG conjugated with Alexa488 (green fluorescence). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20161793), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.
Long Name
C-X-C Motif Chemokine Receptor 4
Entrez Gene IDs
7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names
CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Citations for Human CXCR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancer
    Authors: Tundidor, I;Seijo-Vila, M;Blasco-Benito, S;Rubert-Hern�ndez, M;Ad�mez, S;Andradas, C;Manzano, S;�lvarez-L�pez, I;Sarasqueta, C;Villa-Morales, M;Gonz�lez-Lois, C;Ram�rez-Medina, E;Almoguera, B;S�nchez-L�pez, AJ;Bindila, L;Hamann, S;Arnold, N;R�cken, C;Heras-Murillo, I;Sancho, D;Moreno-Bueno, G;Caffarel, MM;Guzm�n, M;S�nchez, C;P�rez-G�mez, E;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Rapid target validation in a Cas9-inducible hiPSC derived kidney model
    Authors: Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks
    Scientific Reports, 2021-08-16;11(1):16532.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis
    Authors: M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane
    PloS Pathogens, 2021-04-19;17(4):e1009526.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  4. Plasmodium vivax liver stage assay platforms using Indian clinical isolates
    Authors: PA Subramani, N Vartak-Sha, S Sreekumar, P Mathur, B Nayer, S Dakhore, SK Basavanna, DM Kalappa, RV Krishnamur, B Mukhi, P Mishra, N Yoshida, SK Ghosh, R Shandil, S Narayanan, B Campo, K Hasegawa, AR Anvikar, N Valecha, V Sundaramur
    Malar. J., 2020-06-22;19(1):214.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus
    Authors: F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines
    Authors: Mohaned Ahmed, Haneen A. Basheer, Jose M. Ayuso, Djevdet Ahmet, Marco Mazzini, Roshan Patel et al.
    Scientific Reports
  7. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor
    Authors: R Desplantes, C Lévêque, B Muller, M Lotierzo, G Ferracci, M Popoff, M Seagar, R Mamoun, O El Far
    Sci Rep, 2017-04-21;7(1):1032.
    Applications: Surface Plasmon Resonance
  8. Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1 beta and MMP-13
    Authors: Pengcui Li, Jin Deng, Xiaochun Wei, Chathuraka T. Jayasuriya, Jingming Zhou, Qian Chen et al.
    Molecular Medicine Reports
  9. Novel role for NFAT3 in ERK-mediated regulation of CXCR4.
    Authors: Huang, Keven, Kiefer, Christin, Kamal, Adeela
    PLoS ONE, 2014-12-16;9(12):e115249.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014-03-19;192(8):3805-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
    Authors: Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M
    J Immunol, 2014-02-03;192(5):2133-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  12. Production of hepatocyte-like cells from human pluripotent stem cells.
    Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
    Nat Protoc, 2013-01-01;8(2):430-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2012-11-07;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  14. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2011-11-07;72(1):66-75.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. CXCR4 suppression attenuates EGFRvIII-mediated invasion and induces p38 MAPK-dependent protein trafficking and degradation of EGFRvIII in breast cancer cells
    Authors: Massod Rahimi, Theodore A. Toth, Careen K. Tang
    Cancer Letters
  16. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
    Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
    Int. J. Cancer, 2010-03-15;126(6):1302-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. CXCR7 functions as a scavenger for CXCL12 and CXCL11.
    Authors: Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
    PLoS ONE, 2010-02-11;5(2):e9175.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment.
    Authors: Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S
    J. Exp. Med., 2008-12-29;206(1):249-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  19. CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells.
    Authors: Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M
    Infect. Immun., 2008-04-21;76(7):3304-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
    Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
    J. Pathol., 2008-03-01;214(4):464-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007-09-15;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004-12-01;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Factors that increase the effective concentration of CXCR4 dictate feline immunodeficiency virus tropism and kinetics of replication.
    Authors: de Parseval A, Ngo S, Sun P, Elder JH
    J. Virol., 2004-09-01;78(17):9132-43.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.
    Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping
    Nat Med, 2004-08-22;10(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry.
    Authors: Hatse S, Princen K, Vermeire K, Gerlach LO, Rosenkilde MM, Schwartz TW, Bridger G, De Clercq E, Schols D
    FEBS Lett., 2003-07-10;546(2):300-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection.
    Authors: Willett BJ, Cannon CA, Hosie MJ
    J. Virol., 2003-01-01;77(1):709-12.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. CXCR4 function requires membrane cholesterol: implications for HIV infection.
    Authors: Nguyen DH, Taub D
    J. Immunol., 2002-04-15;168(8):4121-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  29. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms.
    Authors: Rahimi Massod, George Jessica, Tang Careen et al.
    International Journal of Cancer

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