Human Cyclin A1 Antibody Summary
Lys51-Arg220
Accession # P78396
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Cyclin A1 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cyclin A1 in Human Testis. Cyclin A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin A1
Cyclin A1, also known as CCNA1, is a 64 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression, apoptosis, double strand DNA break repair, and mRNA splicing. Cyclin A1 activity promotes G2/M phase transition in spermatocyte meiosis and G1/S transition in somatic cells. Cyclin A1 is upregulated in myeloid leukemia, testicular germ cell tumors, and several carcinomas. It contains two cyclin box folds (aa 243-318 and 340-427). Over aa 51-220, human Cyclin A1 shares approximately 75% aa identity with mouse and rat Cyclin A1.
Product Datasheets
Citations for Human Cyclin A1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Targeting SOX18 Transcription Factor Activity by Small-Molecule Inhibitor Sm4 in Non-Small Lung Cancer Cell Lines
Authors: Olga Rodak, Monika Mrozowska, Agnieszka Rusak, Agnieszka Gomułkiewicz, Aleksandra Piotrowska, Mateusz Olbromski et al.
International Journal of Molecular Sciences
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Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells.
Authors: Kim Y, Chen C, Bolton E
PLoS ONE, 2015-09-15;10(9):e0138286.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation
Authors: Yi-Heng Hao, M. Carmen Lafita-Navarro, Lauren Zacharias, Nofit Borenstein-Auerbach, Min Kim, Spencer Barnes et al.
Cell Communication and Signaling
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