Human Cyr61/CCN1 Antibody

Catalog #: MAB40551 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB40551-100
MAB40551-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cyr61/CCN1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cyr61/CCN1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 434311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cyr61/CCN1
Ala22-Asp381
Accession # O00622

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Cyr61/CCN1 DuoSet ELISA Kit (Catalog # DY4055) for convenient development of a sandwich ELISA or the Human Cyr61/CCN1 Quantikine ELISA Kit (Catalog # DCYR10) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Cyr61/CCN1 ELISA Standard Curve. Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyr61/CCN1

Cyr61, also known as CCN1, is a 40 - 45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4 - 6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8 - 12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

References

Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
Jay, P. et al. (1997) Oncogene 14:1753.
Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248.
Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
Xie, D. et al. (2004) Cancer Res. 64:1987.
Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594.
Monnier, Y. et al. (2008) Cancer Res. 68:7323.
Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Alternate Names

CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1