Home / Cyr61/CCN1 / Human Cyr61/CCN1 Antibody

Human Cyr61/CCN1 Antibody

Catalog #: AF6009
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6009
AF6009-SP
Detection of Human Cyr61/CCN1 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cyr61/CCN1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cyr61/CCN1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cyr61/CCN1
Ala22-Asp381
Accession # O00622
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Cyr61/CCN1 DuoSet ELISA Kit (Catalog # DY4055) for convenient development of a sandwich ELISA or the Human Cyr61/CCN1 Quantikine ELISA Kit (Catalog # DCYR10) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cyr61/CCN1 antibody by Western Blot. View Larger

Detection of Human Cyr61/CCN1 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human Cyr61/CCN1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Cyr61/CCN1 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 52 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ELISA Human Cyr61/CCN1 Antibody in ELISA Standard Curve. View Larger

Human Cyr61/CCN1 ELISA Standard Curve. Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyr61/CCN1

Cysteine-rich angiogenic inducer 61 (Cyr61), also known as CCN1, is a 40‑45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4‑6). Plasmin cleavage of
ECM‑bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8‑12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

References
  1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
  2. Jay, P. et al. (1997) Oncogene 14:1753.
  3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
  4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
  5. Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248.
  6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
  7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
  8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
  9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
  10. Xie, D. et al. (2004) Cancer Res. 64:1987.
  11. Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594.
  12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
  13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
  14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
  15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
  16. Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Entrez Gene IDs
3491 (Human); 16007 (Mouse); 83476 (Rat)
Alternate Names
CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1

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Citation for Human Cyr61/CCN1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Antiproliferative and Antimigratory Effects of a Novel YAP–TEAD Interaction Inhibitor Identified Using in Silico Molecular Docking
    Authors: Sarah A. Smith, Richard B. Sessions, Deborah K. Shoemark, Christopher Williams, Reza Ebrahimighaei, Madeleine C. McNeill et al.
    Journal of Medicinal Chemistry

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