Human Cytokeratin 19 Antibody Summary
Applications
Bartek J. et al. (1985) Int J Cancer. 36(3):299. This application was not tested by R&D Systems.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cytokeratin 19 in MDA‑MB‑231 Human Cell Line. Cytokeratin 19 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB3506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Cytokeratin 19 in Human Breast Cancer Tissue. Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB3506) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 19
Cytokeratin 19, a type I intermediate filament protein, is the smallest known acidic cytokeratin. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours.
Product Datasheets
Citations for Human Cytokeratin 19 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
Biomedicines, 2021-12-14;9(12):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture, ELISA Detection -
Inhibition of nitric oxide synthesis during induced cholestasis ameliorates hepatocellular injury by facilitating S-nitrosothiol homeostasis.
Authors: Lopez-Sanchez LM, Corrales FJ, Barcos M, Espejo I, Munoz-Castaneda JR, Rodriguez-Ariza A
Lab. Invest., 2009-10-05;90(1):116-27.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P
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