Human DC-LAMP Antibody

Catalog #: MAB4087 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4087
MAB4087-SP

Detection of Human DC-LAMP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DC-LAMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human DC-LAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAMP is observed.

Source

Monoclonal Mouse IgG_2A Clone # 530217

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DC-LAMP
Asp21-Thr381
Accession # EAW78338

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DC-LAMP by Western Blot. Western blot shows lysates ofin vitrodifferentiated human myeloid mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DC-LAMP Monoclonal Antibody (Catalog # MAB4087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-LAMP

DC-LAMP (dendritic cell lysosome-associated membrane protein; also CD208 and LAMP3) is a member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It can be variably glycosylated, and apparent sizes from 54kDa to 90 kDa have been reported. It has a 254 aa luminal N-terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. It may play a role in normal lysosome and endosome function. Over aa 21‑381, human DC-LAMP shares 72% and 55% aa sequence identity with dog and mouse DC‑LAMP, respectively.

Long Name

Dendritic Cell-lysosome-associated Membrane Glycoprotein

Entrez Gene IDs

27074 (Human); 239739 (Mouse); 303801 (Rat)

Alternate Names

CD208 antigen; CD208; DCLAMP; DC-LAMP; DCLAMPDC-lysosome-associated membrane glycoprotein; LAMP; LAMP3; LAMP-3; lysosomal-associated membrane protein 3DC LAMP; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403; TSC403CD208