Human Dectin-1/CLEC7A APC-conjugated Antibody
Human Dectin-1/CLEC7A APC-conjugated Antibody Summary
Thr66-Met201
Accession # NP_072092
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin-1/CLEC7A APC-conjugated Monoclonal Antibody (Catalog # FAB1859A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
- Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
- Yokota, K. et al. (2001) Gene 272:51.
- Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
- Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
- Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
- Gantner, B.N. et al. (2005) EMBO J. 24:1277.
- Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
- Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
- Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
- Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
- Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
- Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
- Saijo, S. et al. (2007) Nat. Immunol. 8:39.
- Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Product Datasheets
Citations for Human Dectin-1/CLEC7A APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Tetraspanin CD82 Organizes Dectin-1 into Signaling Domains to Mediate Cellular Responses to Candida albicans
Authors: JM Tam, JL Reedy, DP Lukason, SG Kuna, M Acharya, NS Khan, PE Negoro, S Xu, RA Ward, MB Feldman, RA Dutko, JB Jeffery, A Sokolovska, CN Wivagg, KG Lassen, F Le Naour, V Matzaraki, EC Garner, RJ Xavier, V Kumar, FL van de Vee, MG Netea, CK Miranti, MK Mansour, JM Vyas
J. Immunol., 2019-04-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genetic deficiency of NOD2 confers resistance to invasive aspergillosis
Authors: MS Gresnigt, C Cunha, M Jaeger, SM Gonçalves, RKS Malireddi, A Ammerdorff, R Lubbers, M Oosting, O Rasid, G Jouvion, C Fitting, DJ Jong, JF Lacerda, A Campos, WJG Melchers, K Lagrou, J Maertens, TD Kanneganti, A Carvalho, O Ibrahim-Gr, FL van de Vee
Nat Commun, 2018-07-06;9(1):2636.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Absence of NOD1 Enhances Killing of Aspergillus fumigatus Through Modulation of Dectin-1 Expression
Authors: MS Gresnigt, M Jaeger, RK Subbarao M, O Rasid, G Jouvion, C Fitting, WJG Melchers, TD Kanneganti, A Carvalho, O Ibrahim-Gr, FL van de Vee
Front Immunol, 2017-12-13;8(0):1777.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization
Authors: Ofer Levy
J. Immunol., 2016-10-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Impaired T cell responsiveness to interleukin-6 in hematological patients with invasive aspergillosis.
Authors: Camargo J, Bhimji A, Kumar D, Kaul R, Pavan R, Schuh A, Seftel M, Lipton J, Gupta V, Humar A, Husain S
PLoS ONE, 2015-04-02;10(4):e0123171.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity.
Authors: Kleinnijenhuis J, Quintin J, Preijers F, Benn C, Joosten L, Jacobs C, van Loenhout J, Xavier R, Aaby P, van der Meer J, van Crevel R, Netea M
J Innate Immun, 2013-10-30;6(2):152-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Bacille Calmette-Guérin induces NOD2-dependent nonspecific protection from reinfection via epigenetic reprogramming of monocytes
Authors: Johanneke Kleinnijenhuis, Jessica Quintin, Frank Preijers, Leo A. B. Joosten, Daniela C. Ifrim, Sadia Saeed et al.
Proceedings of the National Academy of Sciences
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Detection of human Dectin1 on HEK cells overexpressing isoform A of human Dectin1. The cells were treated with 5 μl of the human Dectin1 APC-conjugated antibody (catalogue # FAB1859A) (BLUE) or mouse IgG2b APC-conjugated isotype control antibody (RED)
