Human Dectin‑1/CLEC7A Biotinylated Antibody

Catalog #: BAF1859 Datasheet / COA / SDS

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BAF1859

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Human Dectin‑1/CLEC7A Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dectin-1/CLEC7A in Western blots. In this format, less than 5% cross-reactivity with recombinant mouse (rm) Dectin‑1, recombinant human Dectin-2, and rmDectin-2 alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A (R&D Systems, Catalog # 1859-DC)
Thr66-Met201
Accession # NP_072092

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Dectin‑1/CLEC7A (Catalog # 1859-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
Yokota, K. et al. (2001) Gene 272:51.
Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
Gantner, B.N. et al. (2005) EMBO J. 24:1277.
Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
Saijo, S. et al. (2007) Nat. Immunol. 8:39.
Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Alternate Names

Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1