Human E-Cadherin APC-conjugated Antibody Summary
Asp155-Ile707
Accession # P12830
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of E-Cadherin in MCF-7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human E-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB18381A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Cadherin
Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
Product Datasheets
Citations for Human E-Cadherin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Modeling of FAN1-Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System
Authors: Lim, SW;Na, D;Lee, H;Fang, X;Cui, S;Shin, YJ;Lee, KI;Lee, JY;Yang, CW;Chung, BH;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
An oncolytic herpesvirus expressing E-cadherin improves survival in mouse models of glioblastoma
Authors: B Xu, R Ma, L Russell, JY Yoo, J Han, H Cui, P Yi, J Zhang, H Nakashima, H Dai, EA Chiocca, B Kaur, MA Caligiuri, J Yu
Nat. Biotechnol., 2018-11-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
Cell Stem Cell, 2009-03-06;4(3):248-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?
The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent.
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