Human EGF Antibody

Catalog #: MAB236
16 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB236-SP
MAB236-100
MAB236-500

Detection of Recombinant Human and Rat EGF by Western Blot.

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All EGF Antibodies

Product Details

Citations (16)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) HB‑EGF or rhTGF‑ alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.

Source

Monoclonal Mouse IgG₁ Clone # 10825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human EGF

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.05-0.1 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry

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EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND₅₀ is typically 0.05-0.1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway
Authors: Antonios Chronopoulos, Stephen D. Thorpe, Ernesto Cortes, Dariusz Lachowski, Alistair J. Rice, Vasyl V. Mykuliak et al.
Nature Materials
E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer
Authors: Ethan L. Morgan, James A. Scarth, Molly R. Patterson, Christopher W. Wasson, Georgia C. Hemingway, Diego Barba-Moreno et al.
Cell Death & Differentiation
Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling
Authors: Bauer, L;Edwards, J;Heil, A;Dewitt, S;Biebermann, H;Aeschlimann, D;Knäuper, V;
International journal of molecular sciences
Species: Human
Sample Types: BALF
Applications: Neutralization
Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts
Authors: E Ticiani, J Gingrich, Y Pu, M Vettathu, J Davis, D Martin, MG Petroff, A Veiga-Lope
Environmental health perspectives, 2021-02-19;129(2):27005.
Species: Human
Sample Types: Complex Sample Type
Applications: Control
VEGFR-1 Regulates EGF-R to Promote Proliferation in Colon Cancer Cells
Authors: H Nagano, C Tomida, N Yamagishi, S Teshima-Ko
Int J Mol Sci, 2019-11-09;20(22):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions
Authors: S Jeong, B Kim, HC Lau, A Kim
Pharmaceutics, 2019-10-14;11(10):.
Species: Human
Sample Types: Coacervates
Applications: Western Blot
Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas
Authors: Y Iwata
Br. J. Dermatol, 2017-08-20;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
Authors: S Müller-Deu, L Seefried, M Krug, F Jakob, R Ebert
Stem Cell Res, 2017-08-18;24(0):69-76.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
Expression and clinical value of EGFR in human meningiomas
Authors: MB Arnli, T Backer-Grø, B Ytterhus, US Granli, S Lydersen, S Gulati, SH Torp
PeerJ, 2017-03-29;5(0):e3140.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
Cancer Res, 2014-03-31;74(10):2869-81.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Photodynamic therapy with 5-aminolevulinic acid (ALA) impairs tumor initiating and chemo-resistance property in head and neck cancer-derived cancer stem cells.
Authors: Yu, Chuan-Ha, Yu, Cheng-Ch
PLoS ONE, 2014-01-24;9(1):e87129.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010-02-19;7(0):13.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P

S100A11, an dual mediator for growth regulation of human keratinocytes.
Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Mol. Biol. Cell, 2007-10-31;19(1):78-85.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot

Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
J. Invest. Dermatol., 2006-05-11;126(9):2096-105.
Species: Human
Sample Types: Serum
Applications: Neutralization

Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
J. Mol. Endocrinol., 2005-04-01;34(2):353-66.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors
Authors: Tamara Muliaditan, Leena Halim, Lynsey M. Whilding, Benjamin Draper, Daniela Y. Achkova, Fahima Kausar et al.
Cell Reports Medicine