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Human EMMPRIN/CD147 ELISA Kit - Quantikine

Catalog #: DEMP00
8 Citations 1 Review Datasheet / COA / SDS
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DEMP00
Control Products Available
QC80: Quantikine Immunoassay Control Set 995 for Human EMMPRIN
Human EMMPRIN/CD147 ELISA Serum/Plasma Standard Curve
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Product Details
Procedure
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human EMMPRIN/CD147 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
9.77 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human EMMPRIN
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human EMMPRIN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human EMMPRIN in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human EMMPRIN and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human EMMPRIN showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring EMMPRIN.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 448 1257 2412 396 1183 2363
Standard Deviation 20.1 43 120 21.1 70.5 120
CV% 4.5 3.4 5 5.3 6 5.1

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 431 1208 2341 421 1240 2474
Standard Deviation 15.3 46.9 109 23.7 54.9 145
CV% 3.5 3.9 4.7 5.6 4.4 5.9

Recovery

The recovery of EMMPRIN spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 91-111
EDTA Plasma (n=4) 98 90-110
Heparin Plasma (n=4) 96 85-104
Serum (n=4) 100 90-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of EMMPRIN were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human EMMPRIN/CD147 ELISA Linearity

Scientific Data

N/A Human EMMPRIN/CD147 ELISA Serum/Plasma Standard Curve View Larger

Human EMMPRIN/CD147 ELISA Serum/Plasma Standard Curve

N/A Human EMMPRIN/CD147 ELISA Cell Culture Supernates/Saliva/Urine/Human Milk Standard Curve View Larger

Human EMMPRIN/CD147 ELISA Cell Culture Supernates/Saliva/Urine/Human Milk Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EMMPRIN/CD147

The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.

Long Name:
Extracellular Matrix Metalloproteinase Inducer
Entrez Gene IDs:
682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)
Alternate Names:
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human EMMPRIN/CD147 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population
    Authors: LY Wang, CS Tan, MKP Lai, S Hilal
    Journal of Clinical Medicine, 2022-12-08;11(24):.
    Species: Human
    Sample Types: Serum
  2. Cluster of Differentiation 147 (CD147) serves as a promoter of atherosclerosis in patients with cerebral infarction
    Authors: GJ Wang, X Dong, Y Li
    European review for medical and pharmacological sciences, 2022-08-01;26(16):5710-5717.
    Species: Human
    Sample Types: Serum
  3. BSG (CD147) Serum Level and Genetic Variants Are Associated with Overall Survival in Acute Myeloid Leukaemia
    Authors: P ?acina, A Butrym, E Turlej, M Stachowicz, J Wietrzyk, G Mazur, K Bogunia-Ku
    Journal of Clinical Medicine, 2022-01-10;11(2):.
    Species: Human
    Sample Types: Serum
  4. CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults
    Authors: MM Ali, I Mirza, D Naquiallah, C Hassan, M Masrur, FM Bianco, AM Mahmoud
    Journal of cardiovascular development and disease, 2021-12-28;9(1):.
    Species: Human
    Sample Types: Plasma
  5. Clinical Significance of CD147 in Children with Inflammatory Bowel Disease
    Authors: H Wang, J Ye, R Liu, G Chen, J Zhao, L Huang, F Yang, M Li, S Zhang, Jingxie, L Xiong, H Chen, Y Xu, M Su, Y Xie, S Li, F Zheng, L Geng, W Xu, S Gong
    Biomed Res Int, 2020-09-15;2020(0):7647181.
    Species: Human
    Sample Types: Serum
  6. Soluble EMMPRIN levels discriminate aortic ectasia in Marfan syndrome patients
    Authors: E Rurali, GL Perrucci, R Gaetano, A Pini, D Moschetta, D Gentilini, P Nigro, G Pompilio
    Theranostics, 2019-04-12;9(8):2224-2234.
    Species: Human
    Sample Types: Plasma
  7. Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism
    Authors: BL Updegraff, X Zhou, Y Guo, MS Padanad, PH Chen, C Yang, J Sudderth, C Rodriguez-, L Girard, JD Minna, P Mishra, RJ DeBerardin, KA O'Donnell
    Cell Rep, 2018-11-20;25(8):2223-2233.e6.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma.
    Authors: Wu J, Hao Z, Zhao Y, Yang X, Tang H, Zhang X, Song F, Sun X, Wang B, Nan G, Chen Z, Bian H
    J Transl Med, 2014-07-04;12(0):190.
    Species: Human
    Sample Types: Serum

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Human EMMPRIN/CD147 Quantikine ELISA Kit
By Marry Tahani on 11/21/2018
Sample Tested: Serum