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Human Endoglin/CD105 APC-conjugated Antibody

Catalog #: FAB10971A
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB10971A-100
FAB10971A-025
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry.
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Citations (11)
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Human Endoglin/CD105 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglin/CD105.
Source
Monoclonal Mouse IgG1 Clone # 166707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
Glu26-Gly586
Accession # Q5T9B9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Endoglin/CD105 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 APC-conjugated Mono-clonal Antibody (Catalog # FAB10971A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2‑5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Human Endoglin/CD105 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells
    Authors: Hai-Bo Huang, Hai-Tao Luo, Na-Na Wei, Miao-Ling Liu, Fei He, Wei Yang et al.
    Stem Cell Research & Therapy
  2. Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery
    Authors: S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi
    Nature Communications, 2021-08-18;12(1):5006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The neovascularization effect of dedifferentiated fat cells
    Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
    Sci Rep, 2020-06-08;10(1):9211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
    Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
    Stem Cells Int, 2019-01-03;2019(0):5690345.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
    Authors: TL Ramos, LI Sánchez-Ab, B Rosón-Burg, A Redondo, A Rico, S Preciado, R Ortega, C Rodríguez, S Muntión, Á Hernández-, J De Las Riv, M González, JR González P, C Del Cañizo, F Sánchez-Gu
    PLoS ONE, 2017-08-10;12(8):e0182470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
    Authors: Muntion S, Ramos T, Diez-Campelo M, Roson B, Sanchez-Abarca L, Misiewicz-Krzeminska I, Preciado S, Sarasquete M, De Las Rivas J, Gonzalez M, Sanchez-Guijo F, Del Canizo M
    PLoS ONE, 2016-02-02;11(2):e0146722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry.
    Authors: L Ramos T, Sanchez-Abarca L, Muntion S, Preciado S, Puig N, Lopez-Ruano G, Hernandez-Hernandez A, Redondo A, Ortega R, Rodriguez C, Sanchez-Guijo F, Del Canizo C
    Cell Commun Signal, 2016-01-12;14(1):2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Effects of long-term hypoxia and pro-survival cocktail in bone marrow-derived stromal cell survival.
    Authors: Kim S, Chaudhry A, Lee I, Frank J
    Stem Cells Dev, 2013-12-20;23(5):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system.
    Authors: Tran NT, Trinh QM, Lee GM
    Stem Cells Dev., 2011-09-27;21(7):1165-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro
    Authors: Xiaoyu Xing, Zhiyuan Zhang, Liang Zhong, Guanqun Ju, Xiangyu Zou, Yingjian Zhu et al.
    Experimental and Therapeutic Medicine
  11. Targeting aberrant DNA methylation in mesenchymal stromal cells as a treatment for myeloma bone disease
    Authors: Antonio Garcia-Gomez, Tianlu Li, Carlos de la Calle-Fabregat, Javier Rodríguez-Ubreva, Laura Ciudad, Francesc Català-Moll et al.
    Nature Communications

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