Human Endoglycan/PODXL2 Biotinylated Antibody

Catalog #: BAF1524 Datasheet / COA / SDS

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BAF1524

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Human Endoglycan/PODXL2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Endoglycan/PODXL2 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PODXL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
Gly33-Thr500
Accession # Q9NZ53

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Endoglycan/PODXL2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 (PODXL2), is a member of the CD34 family of sialomucins. It is a Type I transmembrane protein expressed on endothelial cells, early hematopoietic progenitors and leukocyte subpopulations. Specific endoglycan glycoforms function as L-Selectin ligands.

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Alternate Names

Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2