Human Enolase 2/Neuron-specific Enolase ELISA Kit - Quantikine
Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.15 | 8.41 | 13 | 1.96 | 8.5 | 12.6 |
| Standard Deviation | 0.03 | 0.17 | 0.36 | 0.13 | 0.33 | 0.55 |
| CV% | 1.4 | 2 | 2.8 | 6.7 | 3.9 | 4.3 |
Recovery
The recovery of Enolase 2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 99 | 85-114 |
| Cell Lysates (n=8) | 105 | 85-115 |
| Platelet-poor Heparin Plasma (n=4) | 95 | 85-111 |
| Serum (n=4) | 95 | 85-110 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Enolase 2/Neuron-specific Enolase
Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Enolase 2/Neuron-specific Enolase Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Stress biomarker changes following a series of repeated static and dynamic apneas in non-divers
Authors: Elia, A;Barlow, MJ;Lees, MJ;Petri, G;Keramidas, ME;
Respiratory physiology & neurobiology
Species: Human
Sample Types: Serum
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Blood-Brain Barrier Biomarkers before and after Kidney Transplantation
Authors: L Hernandez, LJ Ward, S Arefin, P Barany, L Wennberg, M Söderberg, S Bruno, V Cantaluppi, P Stenvinkel, K Kublickien
International Journal of Molecular Sciences, 2023-04-01;24(7):.
Species: Human
Sample Types: Plasma
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Cerebrovascular pulsatility index is higher in chronic kidney disease
Authors: ES Oh, KA Freeberg, CN Steele, W Wang, H Farmer-Bai, ME Coppock, DR Seals, M Chonchol, MJ Rossman, DH Craighead, KL Nowak
Physiological Reports, 2023-01-01;11(1):e15561.
Species: Human
Sample Types: Serum
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Blood-brain barrier and gut barrier dysfunction in chronic kidney disease with a focus on circulating biomarkers and tight junction proteins
Authors: L Hernandez, LJ Ward, S Arefin, T Ebert, A Laucyte-Ci, GOING-FWD, O Heimbürger, P Barany, L Wennberg, P Stenvinkel, K Kublickien
Scientific Reports, 2022-03-15;12(1):4414.
Species: Human
Sample Types: Serum
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New Insights on Old Biomarkers Involved in Tumor Microenvironment Changes and Their Diagnostic Relevance in Non-Small Cell Lung Carcinoma
Authors: K Wadowska, P B?asiak, A Rzechonek, I Bil-Lula, M ?liwi?ska-
Biomolecules, 2021-08-13;11(8):.
Species: Human
Sample Types: Serum
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Effects of Kudiezi Injection on Serum Inflammatory Biomarkers in Patients with Acute Cerebral Infarction
Authors: X Liu, X Jin, B Chen, X Liu, X Liang, X Fang, H Wu, X Fu, H Zheng, X Ding, N Duan, Y Zhang
Dis. Markers, 2018-09-02;2018(0):7936736.
Species: Human
Sample Types: Serum
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Cerebral Biomarkers in Women With Preeclampsia Are Still Elevated 1 Year Postpartum
Am J Hypertens, 2016-12-01;0(0):.
Species: Human
Sample Types: Plasma
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