Home / EpCAM/TROP1 / Human EpCAM/TROP‑1 Biotinylated Antibody

Human EpCAM/TROP‑1 Biotinylated Antibody

Catalog #: BAF960
28 Citations Datasheet / COA / SDS
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BAF960
Detection of EpCAM/TROP‑1 in HT-29 cells by Flow Cytometry
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Citations (28)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EpCAM/TROP‑1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EpCAM/TROP-1 in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, recombinant mouse MAdCAM-1, rhNCAM-1, and rhNCAM-L1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EpCAM, extracelllular domain
Gln24-Lys265
Accession # CAA32870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EpCAM/TROP‑1 Fc Chimera (Catalog # 960-EP)
Flow Cytometry
2.5 µg/106 cells
HT‑29 human colon adenocarcinoma cell line

Human EpCAM/TROP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human
EpCAM/TROP‑1 Antibody (Catalog # MAB9601)

Standard: Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 960-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of
EpCAM/TROP‑1 in HT-29 cells by Flow Cytometry HT-29 cells were stained with Goat Anti-Human
EpCAM/TROP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF960, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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Citations for Human EpCAM/TROP‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Nanoporous micro-element arrays for particle interception in microfluidic cell separation
    Authors: Grace D. Chen, Fabio Fachin, Elena Colombini, Brian L. Wardle, Mehmet Toner
    Lab on a Chip
  2. An anti-EpCAM antibody EpAb2-6 for the treatment of colon cancer
    Authors: Mei-Ying Liao, Jun-Kai Lai, Mark Yen-Ping Kuo, Ruei-Min Lu, Cheng-Wei Lin, Ping-Chang Cheng et al.
    Oncotarget
  3. Surface Functionalized Graphene Biosensor on Sapphire for Cancer Cell Detection
    Authors: Daniel J. Joe, Jeonghyun Hwang, Christelle Johnson, Ho-Young Cha, Jo-Won Lee, Xiling Shen et al.
    Journal of Nanoscience and Nanotechnology
  4. RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
    Authors: Yu M, Ting DT, Stott SL et al.
    Nature
  5. MAPK7 Regulates EMT Features and Modulates the Generation of CTCs
    Authors: Sarah Javaid, Jianmin Zhang, Gromoslaw A. Smolen, Min Yu, Ben S. Wittner, Anurag Singh et al.
    Molecular Cancer Research
  6. A Biopolymer System for Cell Recovery from Microfluidic Cell Capture Devices
    Authors: Ajay M. Shah, Min Yu, Zev Nakamura, Jordan Ciciliano, Matthew Ulman, Kenneth Kotz et al.
    Analytical Chemistry
  7. μ‐opioid receptor agonist facilitates circulating tumor cell formation in bladder cancer via the MOR/AKT/Slug pathway: a comprehensive study including randomized controlled trial
    Authors: Xiaoqiang Wang, Song Zhang, Di Jin, Jiamei Luo, Yumiao Shi, Yiqi Zhang et al.
    Cancer Communications
  8. Multifunctional Gelatin-Nanoparticle-Modified Chip for Enhanced Capture and Non-Destructive Release of Circulating Tumor Cells
    Authors: L Xu, T Ma, K Zhang, Q Zhang, M Yu, X Zhao
    Micromachines, 2022-02-28;13(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  9. Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells
    Authors: H Shen, R Su, J Peng, L Zhu, K Deng, Q Niu, Y Song, L Yang, L Wu, Z Zhu, C Yang
    Bioactive materials, 2021-10-05;11(0):32-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Capture
  10. The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules
    Authors: C Liu, H Chen, T Sun, H Wang, B Chen, X Wang
    Translational Oncology, 2021-03-02;14(5):101052.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. High-purity isolation of rare single cells from blood using a tiered microchip system
    Authors: O Gur, CL Chang, R Jain, Y Zhong, CA Savran
    PLoS ONE, 2020-03-17;15(3):e0229949.
    Species: Human
    Sample Types: Whole Blood
    Applications: ELISA Capture
  12. High-Throughput Label-Free Isolation of Heterogeneous Circulating Tumor Cells and CTC Clusters from Non-Small-Cell Lung Cancer Patients
    Authors: M Zeinali, M Lee, A Nadhan, A Mathur, C Hedman, E Lin, R Harouaka, MS Wicha, L Zhao, N Palanisamy, M Hafner, R Reddy, GP Kalemkeria, BJ Schneider, KA Hassan, N Ramnath, S Nagrath
    Cancers (Basel), 2020-01-03;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. A temporary indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells
    Authors: TH Kim, Y Wang, CR Oliver, DH Thamm, L Cooling, C Paoletti, KJ Smith, S Nagrath, DF Hayes
    Nat Commun, 2019-04-01;10(1):1478.
    Species: Xenograft
    Sample Types: In Vivo
    Applications: Cell Capture
  14. Emerin deregulation links nuclear shape instability to metastatic potential
    Authors: M Reis-Sobre, JF Chen, T Novitskaya, S You, S Morley, K Steadman, N Kaur Gill, A Eskaros, M Rotinen, CY Chu, LWK Chung, H Tanaka, W Yang, BS Knudsen, HR Tseng, AC Rowat, EM Posadas, A Zijlstra, D Di Vizio, MR Freeman
    Cancer Res., 2018-08-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Capture
  15. Photo and Thermo Dual-Responsive Copolymer Surfaces for Efficient Cell Capture and Release
    Authors: Y Hao, H Liu, G Li, H Cui, L Jiang, S Wang
    Chemphyschem, 2018-04-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Tumor-Derived Exosomal Long Noncoding RNAs as Promising Diagnostic Biomarkers for Prostate Cancer
    Authors: YH Wang, J Ji, BC Wang, H Chen, ZH Yang, K Wang, CL Luo, WW Zhang, FB Wang, XL Zhang
    Cell. Physiol. Biochem., 2018-03-26;46(2):532-545.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
    Authors: H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho
    Theranostics, 2018-01-01;8(2):505-517.
    Applications: Functional Assay
  18. Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip
    Authors: MH Park, E Reátegui, W Li, SN Tessier, KH Wong, AE Jensen, V Thapar, D Ting, M Toner, SL Stott, PT Hammond
    J. Am. Chem. Soc., 2017-02-09;139(7):2741-2749.
    Species: N/A
    Sample Types: Protein
    Applications: Cell Selection
  19. Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial mesenchymal transition of gastric cancer
    Aging (Albany NY), 2016-09-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  20. Circulating tumour cells as a biomarker for diagnosis and staging in pancreatic cancer
    Authors: J S Ankeny
    Br J Cancer, 2016-06-14;114(12):1367-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: NanoVelcro Chip
  21. Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
    Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
    BMC Cancer, 2015-05-07;15(0):372.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  22. Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential.
    Authors: Bryan, R T, Shimwell, N J, Wei, W, Devall, A J, Pirrie, S J, James, N D, Zeegers, M P, Cheng, K K, Martin, A, Ward, D G
    Br J Cancer, 2013-11-28;110(3):679-85.
    Species: Human
    Sample Types: Urine
    Applications: Immunoprecipitation, Western Blot
  23. Molecular biomarker analyses using circulating tumor cells.
    Authors: Punnoose EA, Atwal SK, Spoerke JM
    PLoS ONE, 2010-09-08;5(9):e12517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Circulating tumor cells are transcriptionally similar to the primary tumor in a murine prostate model.
    Authors: Helzer KT, Barnes HE, Day L, Harvey J, Billings PR, Forsyth A
    Cancer Res., 2009-09-29;69(19):7860-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  25. High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
    Authors: Burger PE, Gupta R, Xiong X, Ontiveros CS, Salm SN, Moscatelli D, Wilson EL
    Stem Cells, 2009-09-01;27(9):2220-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Isolation of rare circulating tumour cells in cancer patients by microchip technology.
    Authors: Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M
    Nature, 2007-12-20;450(7173):1235-9.
    Species: Human
    Sample Types: Whole Cells
  27. Ultrasensitive Detection of Low-Abundance Surface-Marker Protein Using Isothermal Rolling Circle Amplification in a Microfluidic Nanoliter Platform
    Authors: Tania Konry, Irina Smolina, Joel M. Yarmush, Daniel Irimia, Martin L. Yarmush
    Small
  28. Fluid nanoporous microinterface enables multiscale-enhanced affinity interaction for tumor-derived extracellular vesicle detection
    Authors: Q Niu, J Gao, K Zhao, X Chen, X Lin, C Huang, Y An, X Xiao, Q Wu, L Cui, P Zhang, L Wu, C Yang
    Proceedings of the National Academy of Sciences of the United States of America, 2022-10-28;119(44):e2213236119.

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