Human ErbB2/Her2 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB1129R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1129R-025

Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human ErbB2/Her2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ErbB2/Her2 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.

Source

Monoclonal Mouse IgG_2B Clone # 191924

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1129R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of ErbB2/Her2 in MDA-MB-453 Human Cell Line by Flow Cytometry. MDA-MB-453 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1129R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (Human Epidermal Growth Factor Receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-Kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-Catenin and Plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

References

Coussens, L. et. al. (1985) Science 230:1132.
Yamamoto, T. et. al. (1986) Nature 319:230.
Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
Carraway, K.L. 3^rd et. al. (1994) J. Biol. Chem. 269:14303.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
Schlessinger, J. (2000) Cell 103:211.
Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Alternate Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)