Human ErbB3/Her3 ELISA Kit - Quantikine

Name: Human ErbB3/Her3 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DERB30
Rating: 5 (1 reviews)

Catalog #: DERB30 1 Review Datasheet / COA / SDS

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DERB30

Control Products Available

QC275: Quantikine Immunoassay Control 1035 for Human ErbB3/Her3

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human ErbB3/Her3 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (5 ug), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL), Human Milk (10 uL)

Sensitivity

15.2 pg/mL

Assay Range

62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human ErbB3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Human ErbB3/Her3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB3 in cell culture supernates, cell lysates, serum, plasma, saliva, urine and human milk. It contains NS0-expressed recombinant human ErbB3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB3 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ErbB3.

Recovery

The recovery of human ErbB3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	92-100
Cell Lysis Buffer (n=2)	98	96-101
EDTA Plasma (n=4)	97	93-103
Heparin Plasma (n=4)	96	91-102
Human Milk (n=4)	96	91-103
Saliva (n=4)	100	94-106
Serum (n=4)	100	93-109
Urine (n=4)	97	94-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human ErbB3/Her3 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.

Long Name:

Receptor Tyrosine Protein Kinase ErbB3

Entrez Gene IDs:

2065 (Human); 13867 (Mouse)

Alternate Names:

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)