Human ErbB4/Her4 APC-conjugated Antibody

Catalog #: FAB11311A
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB11311A

Detection of ErbB4/Her4 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human ErbB4/Her4 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ErbB4/Her4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) EGF R, rhErbB2, or rhErbB3 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 182818

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ErbB4/Her4
Glu26-Arg649
Accession # Q15303

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ErbB4/Her4 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB4/Her4 APC-conjugated Monoclonal Antibody (Catalog # FAB11311A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB4/Her4

ErbB4, also called Her4 (human epidermal growth factor receptor 4), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme, ADAM17) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25 aa signal sequence, a 626 aa extracellular domain, a 24 aa transmembrane region, and a 633 aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

References

Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746.
Elenius, K. et al. (1997) J. Biol. Chem. 272:26761.
Elenius, K. et al. (1999) Oncogene 18:2607.
Rio, C. et al. (2000) J. Biol. Chem. 275:10379.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Sundaresan, S. et al. (1998) Endocrinology 139:4756.
Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
Schlessinger, J. (2000) Cell 103:211.
Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB4

Entrez Gene IDs

2066 (Human); 13869 (Mouse); 59323 (Rat)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; EC 2.7.10; EC 2.7.10.1; ErbB4; HER4; HER4MGC138404; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Product Datasheets

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Citations for Human ErbB4/Her4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Molecular Correlates ofIn VitroResponses to Dacomitinib and Afatinib in Bladder Cancer
Authors: S Tamura, Y Wang, B Veeneman, D Hovelson, A Bankhead, LJ Broses, G Lorenzatti, M Liebert, JR Rubin, KC Day, M Hussain, N Neamati, S Tomlins, PL Palmbos, P Grivas, ML Day
Bladder Cancer, 2018-01-20;4(1):77-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
PLoS ONE, 2014-01-29;9(1):e84417.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin
Authors: Ana C. Parente-Pereira, Lynsey M. Whilding, Nancy Brewig, Sjoukje J. C. van der Stegen, David M. Davies, Scott Wilkie et al.
The Journal of Immunology