Human ETV6 Antibody

Catalog #: AF7945
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7945
AF7945-SP

Detection of Human ETV6 by Western Blot.

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All ETV6 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ETV6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ETV6 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ETV6
Ser2-Asp111
Accession # P41212

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ETV6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 15 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ETV6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ETV6 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ETV6

ETV6 (ETS Translocation Variant 6; also TEL [Translocated ETS Leukemia]) is a 58-62 kDa member of the ETS (E-Twenty Six avian leukemia virus) family of proteins. It belongs to a particular class of repressors that are controlled by cell-cycle dependent signals. ETV6 is ubiquitously expressed, and acts as a sequence-specific transcriptional repressor of ETS-binding site-containing gene promoters. It likely does this by recruiting histone deacetylase to its binding site. It is also involved in steroid signaling, and is reported to bind to the DNA binding domains of both RAR and RXR. ETV6 appears to be important in coordinating bone marrow-mediated hematopoiesis. Genes known to be responsive to ETV6 regulation include FLI1, IDI and MMP-3. Human ETV6 is 452 amino acids (aa) in length. It contains one PNT (pointed)/B domain that mediates oligomerization (aa 40-124) followed by an ETS domain that binds DNA (aa 338-422). There are five known utilized Thr/Ser phosphorylation sites plus at least one alternative start site at Met43. This short form is very effective at gene repression due to the absence of a SUMOylation site at Lys11 that, when occupied, blocks DNA binding. Although ETV6 activity is dependent upon homooligomerization, it is also known to bind to TEL2 and FLI1. ETS genes are often involved in chromosomal translocations. ETV6 itself is associated with multiple rearrangements, including the genes encoding AML1, PDGFR beta and BTL (Brx-like Translocated in Leukemia). The translocated sequences of ETV6 differ from chimera to chimera. For example, aa 1-336 are fused to a portion of AML1, aa 1-154 are fused to a portion of PDGFR beta, and aa 12-433 are fused to a portion of BTL. Over aa 2-111, human ETV6 shares 91% aa sequence identity with mouse ETV6.

Long Name

ETS Translocation Variant 6

Entrez Gene IDs

2120 (Human); 14011 (Mouse); 312777 (Rat)

Alternate Names

ETS translocation variant 6; ets variant 6; ets variant gene 6 (TEL oncogene); ETS-related protein Tel1; ETV6; TEL; TEL/ABL; TEL1; TELTEL1 oncogene; transcription factor ETV6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ETV6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Germline ETV6 Mutations Confer Susceptibility to Acute Lymphoblastic Leukemia and Thrombocytopenia.
Authors: Topka S, Vijai J, Walsh M, Jacobs L, Maria A, Villano D, Gaddam P, Wu G, McGee R, Quinn E, Inaba H, Hartford C, Pui C, Pappo A, Edmonson M, Zhang M, Stepensky P, Steinherz P, Schrader K, Lincoln A, Bussel J, Lipkin S, Goldgur Y, Harit M, Stadler Z, Mullighan C, Weintraub M, Shimamura A, Zhang J, Downing J, Nichols K, Offit K
PLoS Genet, 2015-06-23;11(6):e1005262.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot