Human FABP3/H-FABP Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1678
FABP3/H‑FABP in Human Heart.
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Human FABP3/H-FABP Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP3 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) FABP4 is observed, 20% cross-reactivity with recombinant rat (rr) FABP1 is observed, and 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP3
Met1-Ala133
Accession # P05413
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FABP3
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of human fetal brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

FABP3/H‑FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human FABP3/H-FABP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP3/H-FABP

Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.

Long Name
Fatty Acid-Binding Protein 3
Entrez Gene IDs
2170 (Human)
Alternate Names
FABP11; FABP3; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP

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