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Human FABP5/E-FABP Antibody

Catalog #: AF3077
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3077
AF3077-SP
Detection of Human FABP5/E‑FABP by Western Blot.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP5/E-FABP Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10-12.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
FABP5/E‑FABP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FABP5/E‑FABP knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FABP5/E-FABP antibody by Western Blot. View Larger

Detection of Human FABP5/E‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FABP5/E-FABP antibody in HUVEC Human Umbilical Vein Endothelial Cells by Immunocytochemistry (ICC). View Larger

FABP5/E‑FABP in HUVEC Human Umbilical Vein Endothelial Cells. FABP5/E-FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of Human FABP5/E-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E-FABP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human FABP5/E-FABP antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E-FABP at approximately 21 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human FABP5/E-FABP Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FABP5/E‑FABP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP5/E-FABP

Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Long Name
Fatty Acid-Binding Protein 5
Entrez Gene IDs
2171 (Human); 16592 (Mouse); 140868 (Rat)
Alternate Names
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog

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Citations for Human FABP5/E-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Genetic Ablation of the Fatty Acid–Binding Protein FABP5 Suppresses HER2-Induced Mammary Tumorigenesis
    Authors: Liraz Levi, Glenn Lobo, Mary Kathryn Doud, Johannes von Lintig, Darcie Seachrist, Gregory P. Tochtrop et al.
    Cancer Research
  2. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma
    Authors: Chen P, Zhao D, Li J et al.
    Cancer Cell
  3. A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models
    Authors: A Cheng, W Jia, I Kawahata, K Fukunaga
    EBioMedicine, 2021-10-05;72(0):103582.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  4. Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice
    Authors: Q Guo, I Kawahata, T Degawa, Y Ikeda-Mats, M Sun, F Han, K Fukunaga
    Biomedicines, 2021-05-10;9(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Epidermal Fatty Acid-Binding Protein 5 (FABP5) Involvement in Alpha-Synuclein-Induced Mitochondrial Injury under Oxidative Stress
    Authors: Y Wang, Y Shinoda, A Cheng, I Kawahata, K Fukunaga
    Biomedicines, 2021-01-22;9(2):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes
    Authors: A Cheng, I Kawahata, K Fukunaga
    Biomedicines, 2020-12-20;8(12):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  7. Curcumin restores sensitivity to retinoic acid in triple negative breast cancer cells.
    Authors: Thulasiraman P, McAndrews D, Mohiudddin I
    BMC Cancer, 2014-09-27;14(0):724.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.
    Authors: Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
    Hippocampus, 2011-02-01;21(2):162-71.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  9. Fatty acid-binding protein 5 and PPARbeta/delta are critical mediators of epidermal growth factor receptor-induced carcinoma cell growth.
    Authors: Kannan-Thulasiraman P, Seachrist DD, Mahabeleshwar GH, Jain MK, Noy N
    J. Biol. Chem., 2010-04-27;285(25):19106-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Proteogenomic analysis of psoriasis reveals discordant and concordant changes in mRNA and protein abundance
    Authors: William R. Swindell, Henriette A. Remmer, Mrinal K. Sarkar, Xianying Xing, Drew H. Barnes, Liza Wolterink et al.
    Genome Medicine
  11. Saturated fatty acids regulate retinoic acid signalling and suppress tumorigenesis by targeting fatty acid-binding protein 5
    Authors: Liraz Levi, Zeneng Wang, Mary Kathryn Doud, Stanley L. Hazen, Noa Noy
    Nature Communications

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