Human Fc gamma RIII (CD16) Antibody

Detection of Fc gamma RIIIA/B (CD16a/b) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of Human Fc gamma RIII (CD16) by Functional Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung cancer, and 3 gastric cancer) were determined by FCM. A representative graph is shown in (A). The relationship between ULBP3 expression and infiltrating NK cells in tumor tissues is shown in (B). Normal NK cells were co-cultured with CD133−SW620 (C) or 7721 (D) cells at the indicated E:T ratios, and cytotoxicity was measured by LDH release after 6 h of co-culture, with or without the addition of isotype IgG, anti-ULBP3 (B2-F1-F1), and/or anti-CD16 (5 μg/ml). K562 (E) or A549 (F) cells were co-cultured with normal NK cells, and cytotoxicity was measured by LDH release after 6 h of co-culture at the indicated E:T ratios, with or without the addition of ULBP3-Fc (1 ng/ml) and/or B2-F1-F1 (5 μg/ml). The data are representative of results obtained from at least 3 independent experiments. The results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep06138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Fc gamma RIII (CD16) by Functional Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung cancer, and 3 gastric cancer) were determined by FCM. A representative graph is shown in (A). The relationship between ULBP3 expression and infiltrating NK cells in tumor tissues is shown in (B). Normal NK cells were co-cultured with CD133−SW620 (C) or 7721 (D) cells at the indicated E:T ratios, and cytotoxicity was measured by LDH release after 6 h of co-culture, with or without the addition of isotype IgG, anti-ULBP3 (B2-F1-F1), and/or anti-CD16 (5 μg/ml). K562 (E) or A549 (F) cells were co-cultured with normal NK cells, and cytotoxicity was measured by LDH release after 6 h of co-culture at the indicated E:T ratios, with or without the addition of ULBP3-Fc (1 ng/ml) and/or B2-F1-F1 (5 μg/ml). The data are representative of results obtained from at least 3 independent experiments. The results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep06138), licensed under a CC-BY license. Not internally tested by R&D Systems.